Reference - Detail
| RRC ID | 1983 |
|---|---|
| Author | Wu WK, Li GR, Wong HP, Hui MK, Tai EK, Lam EK, Shin VY, Ye YN, Li P, Yang YH, Luo JC, Cho CH. |
| Title | Involvement of Kv1.1 and Nav1.5 in proliferation of gastric epithelial cells. |
| Journal | J Cell Physiol |
| Abstract |
In the present study, patch clamp experiments demonstrated the expression of multiple ionic currents, including a Ba2+-sensitive inward rectifier K+ current (IKir), a 4-aminopyridine- (4-AP) sensitive delayed rectifier K+ current (IKDR), and a nifedipine-sensitive, tetrodotoxin-resistant inward Na+ current (INa.TTXR) in the non-transformed rat gastric epithelial cell line RGM-1. RT-PCR revealed molecular identities of mRNAs for the functional ionic currents, including Kir1.2 for IKir, Kv1.1, Kv1.6, and Kv2.1 for IKDR, and Nav1.5 for INa.TTXR. Pharmacologic blockade of Kv and Nav, but not Kir, suppressed RGM-1 cell proliferation. To further elucidate which subtypes of the ion channels were involved in cell proliferation, RNA interference was employed to knockdown specific gene expression. Downregulation of Kv1.1 or Nav1.5 by RNA interference suppressed RGM-1 cell proliferation. To conclude, our study is the first to delineate the expression of ion channels and their functions as growth modulators in gastric epithelial cells. |
| Volume | 207(2) |
| Pages | 437-44 |
| Published | 2006-5-1 |
| DOI | 10.1002/jcp.20576 |
| PMID | 16331678 |
| MeSH | 4-Aminopyridine / pharmacology Animals Barium / pharmacology Cell Line Cell Proliferation / drug effects* Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / physiology* Gastric Mucosa / cytology Gene Expression / genetics Kv1.1 Potassium Channel / genetics Kv1.1 Potassium Channel / physiology* NAV1.5 Voltage-Gated Sodium Channel Nifedipine / pharmacology Patch-Clamp Techniques Potassium / metabolism Potassium Channels / genetics Potassium Channels / physiology RNA, Small Interfering / genetics Rats Sodium / metabolism Sodium Channels / genetics Sodium Channels / physiology* |
| IF | 5.546 |
| Times Cited | 21 |
| WOS Category | PHYSIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RGM1(RCB0876) |