論文 - 詳細
| RRC ID | 1989 |
|---|---|
| 著者 | Hatao F, Muroi M, Hiki N, Ogawa T, Mimura Y, Kaminishi M, Tanamoto K. |
| タイトル | Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein. |
| ジャーナル | J Leukoc Biol |
| Abstract |
Interleukin-1 receptor-associated kinase (IRAK)-4 is a key mediator in the Toll-like receptor (TLR) signaling. We found that stimulation of TLR2, TLR4, or TLR9, but not TLR3, caused a decrease in IRAK-4 protein without affecting its mRNA level in a mouse macrophage cell line, RAW 264. The decrease in IRAK-4 was accompanied by the appearance of a smaller molecular weight protein (32 kD), which was recognized by an anti-IRAK-4 antibody raised against the C-terminal region. The decrease in IRAK-4 and the appearance of the 32-kD protein occurred with slower kinetics than the activation of IRAK-1 and were suppressed by inhibitors of the proteasome, inducible inhibitor of kappaBalpha phosphorylation or protein synthesis, but not by caspase inhibitors. These results indicate that prolonged stimulation of TLR2, TLR4, or TLR9 causes a down-regulation of IRAK-4 protein, which may be mediated through cleavage of IRAK-4 by a protease induced by the activation of nuclear factor-kappaB. |
| 巻・号 | 76(4) |
| ページ | 904-8 |
| 公開日 | 2004-10-1 |
| DOI | 10.1189/jlb.0504277 |
| PII | jlb.0504277 |
| PMID | 15258191 |
| MeSH | Animals Caspase Inhibitors Caspases / metabolism DNA-Binding Proteins / metabolism* Down-Regulation / drug effects Enzyme Inhibitors / pharmacology I-kappa B Proteins / metabolism Interleukin-1 Receptor-Associated Kinases Lipopolysaccharides / pharmacology Macrophages / metabolism* Membrane Glycoproteins / metabolism* Mice NF-KappaB Inhibitor alpha NF-kappa B / metabolism Phosphorylation / drug effects Phosphotransferases (Alcohol Group Acceptor) / metabolism* Protein Kinases / metabolism Receptors, Cell Surface / metabolism* Toll-Like Receptor 2 Toll-Like Receptor 3 Toll-Like Receptor 4 Toll-Like Receptor 9 Toll-Like Receptors |
| IF | 3.757 |
| 引用数 | 36 |
| WOS 分野 | HEMATOLOGY IMMUNOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |