論文 - 詳細
| RRC ID | 1990 |
|---|---|
| 著者 | Kamata K, Shinozaki K, Okada M, Seto Y, Kobayashi S, Sakae K, Oseto M, Natori K, Shirato-Horikoshi H, Katayama K, Tanaka T, Takeda N, Taniguchi K. |
| タイトル | Expression and antigenicity of virus-like particles of norovirus and their application for detection of noroviruses in stool samples. |
| ジャーナル | J Med Virol |
| Abstract |
Human noroviruses (NoVs), members of the genus Norovirus in the family Caliciviridae, are the leading agents of nonbacterial acute gastroenteritis worldwide. Human NoVs are currently divided into at least two genogroups, genogroup I (GI) and genogroup II (GII), each of which contains at least 14 and 17 genotypes. To explore the genetic and antigenic relationship among NoVs, we expressed the capsid protein of four genetically distinct NoVs, the GI/3 Kashiwa645 virus, the GII/3 Sanbu809 virus, the GII/5 Ichikawa754 virus, and the GII/7 Osaka10-25 virus in baculovirus expression system. An antigen enzyme-linked immunosorbent assay (ELISA) with hyperimmune serum against the four recombinant capsid proteins and characterized previously three capsid proteins derived from GI/1, GI/4, and GII/12 was developed to detect the NoVs antigen in stools. The antigen ELISA was highly specific to the homotypic strains, allowing assignment of a strain to a Norovirus genetic cluster within a genogroup. |
| 巻・号 | 76(1) |
| ページ | 129-36 |
| 公開日 | 2005-5-1 |
| DOI | 10.1002/jmv.20334 |
| PMID | 15778983 |
| MeSH | Animals Antigens, Viral / analysis* Antigens, Viral / biosynthesis Antigens, Viral / genetics Baculoviridae / genetics Baculoviridae / metabolism Caliciviridae Infections / diagnosis Capsid Proteins / analysis Capsid Proteins / biosynthesis Capsid Proteins / genetics Enzyme-Linked Immunosorbent Assay / methods Feces / virology* Gastroenteritis / diagnosis Humans Norovirus / genetics Norovirus / immunology Norovirus / isolation & purification* Phylogeny Recombinant Proteins / biosynthesis |
| IF | 2.021 |
| 引用数 | 18 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |