RRC ID 19969
著者 Nakamura M, Yoshizaki F, Sugimura Y.
タイトル Accumulation of plastocyanin mRNA lacking 5' region in the green alga Pediastrum boryanum grown under copper-deficient conditions.
ジャーナル Plant Cell Physiol
Abstract In the green alga Pediastrum boryanum NIES-301, plastocyanin accumulates under copper-sufficient conditions and cytochrome c6 accumulates under copper-deficient conditions. We cloned the cDNA which encodes pre-apoplastocyanin from P. boryanum cultured under the copper-sufficient condition. The deduced amino acid sequence of the pre-apoplastocyanin protein consists of 151 amino acid residues including a putative bipartite presequence of 53 amino acid residues. Southern blot analysis of P. boryanum genomic DNA indicated that pre-apoplastocyanin is encoded by a single nuclear gene. Northern blot analysis showed that copper-deficient cells accumulated a shorter form of the mRNA of pre-apoplastocyanin, which did not generate pre-apoplastocyanin in the wheat-germ translation system. The difference in size was ascribed to the absence of the 5' region in the mRNA of pre-apoplastocyanin obtained from the copper-deficient cells, which accounts for the absence of plastocyanin under these conditions. This phenomenon represents a novel regulatory mechanism, although details of the mechanism are not yet known.
巻・号 41(1)
ページ 33-41
公開日 2000-1-1
DOI 10.1093/pcp/41.1.33
PMID 10750706
MeSH Algal Proteins / genetics Algal Proteins / metabolism Amino Acid Sequence Base Sequence Chlorophyta / drug effects* Chlorophyta / genetics Copper / pharmacology* DNA / chemistry DNA / genetics DNA, Complementary / chemistry DNA, Complementary / genetics Dose-Response Relationship, Drug Genes / genetics Introns Molecular Sequence Data Oxidation-Reduction Plastocyanin / genetics* Plastocyanin / metabolism Protein Precursors / genetics RNA, Messenger / drug effects* RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Analysis, DNA
IF 4.062
引用数 6
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
藻類 NIES-301