RRC ID |
19969
|
著者 |
Nakamura M, Yoshizaki F, Sugimura Y.
|
タイトル |
Accumulation of plastocyanin mRNA lacking 5' region in the green alga Pediastrum boryanum grown under copper-deficient conditions.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
In the green alga Pediastrum boryanum NIES-301, plastocyanin accumulates under copper-sufficient conditions and cytochrome c6 accumulates under copper-deficient conditions. We cloned the cDNA which encodes pre-apoplastocyanin from P. boryanum cultured under the copper-sufficient condition. The deduced amino acid sequence of the pre-apoplastocyanin protein consists of 151 amino acid residues including a putative bipartite presequence of 53 amino acid residues. Southern blot analysis of P. boryanum genomic DNA indicated that pre-apoplastocyanin is encoded by a single nuclear gene. Northern blot analysis showed that copper-deficient cells accumulated a shorter form of the mRNA of pre-apoplastocyanin, which did not generate pre-apoplastocyanin in the wheat-germ translation system. The difference in size was ascribed to the absence of the 5' region in the mRNA of pre-apoplastocyanin obtained from the copper-deficient cells, which accounts for the absence of plastocyanin under these conditions. This phenomenon represents a novel regulatory mechanism, although details of the mechanism are not yet known.
|
巻・号 |
41(1)
|
ページ |
33-41
|
公開日 |
2000-1-1
|
DOI |
10.1093/pcp/41.1.33
|
PMID |
10750706
|
MeSH |
Algal Proteins / genetics
Algal Proteins / metabolism
Amino Acid Sequence
Base Sequence
Chlorophyta / drug effects*
Chlorophyta / genetics
Copper / pharmacology*
DNA / chemistry
DNA / genetics
DNA, Complementary / chemistry
DNA, Complementary / genetics
Dose-Response Relationship, Drug
Genes / genetics
Introns
Molecular Sequence Data
Oxidation-Reduction
Plastocyanin / genetics*
Plastocyanin / metabolism
Protein Precursors / genetics
RNA, Messenger / drug effects*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Analysis, DNA
|
IF |
4.062
|
引用数 |
6
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
藻類 |
NIES-301 |