| RRC ID |
20053
|
| Author |
Okamoto T, Kim D, Oda T, Matsuoka K, Ishimatsu A, Muramatsu T.
|
| Title |
Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigma akashiwo.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Chattonella marina and Heterosigma akashiwo, known as red tide phytoplankton, are naturally wall-less and have quite fragile cell structures. In this study, we found that an equilibrium dialysis technique allowed the study of lectin binding to these flagellates. The results suggested that concanavalin A (Con A) binds to these flagellate cells through the specific carbohydrate moieties on the cell surface. Interestingly, the binding of an excess of Con A on the cell surface caused morphological changes concomitant with discharge of glycocalyx, a polysaccharide-containing common structure on the external cell surface of these flagellates. Fluorescent microscopic observation using FITC-labeled Con A (F-Con A) confirmed that F-Con A molecules are localized on the discharged glycocalyx.
|
| Volume |
64(8)
|
| Pages |
1767-70
|
| Published |
2000-8-1
|
| DOI |
10.1271/bbb.64.1767
|
| PMID |
10993174
|
| MeSH |
Animals
Concanavalin A / pharmacology*
Dinoflagellida / drug effects*
Dinoflagellida / metabolism
Fluorescein-5-isothiocyanate
Glycocalyx / metabolism*
Microscopy, Fluorescence
Phytoplankton / drug effects*
Phytoplankton / metabolism
Surface Properties
|
| IF |
1.516
|
| Times Cited |
8
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Algae |
NIES-6 |
