RRC ID 2006
Author Tamai H, Iwabuchi M, Meshi T.
Title Arabidopsis GARP transcriptional activators interact with the Pro-rich activation domain shared by G-box-binding bZIP factors.
Journal Plant Cell Physiol.
Abstract The Pro-rich regions, found in a subset of plant bZIP transcription factors, including G-box-binding factors (GBFs) of Arabidopsis thaliana, are thought to be deeply involved in transcriptional regulation. However, the molecular mechanisms of the Pro-rich region-mediated transcriptional regulation are still largely unknown. Here we report evidence showing that two closely related Arabidopsis proteins, designated GPRI1 and GPRI2, containing a GARP DNA-binding domain, are likely partners of one or more GBFs. The results of yeast two-hybrid assays and in vitro binding assays indicated that GPRI1 can interact with the Pro-rich regions of GBF1 and GBF3. GPRI2 interacted with the Pro-rich region of GBF1. GPRI1 and GPRI2 transactivated transcription in yeast. In GPRI1 the region responsible for this activation was mapped in the N-terminal third of the protein. Transient assays showed that in Arabidopsis cells not only the N-terminal but also the C-terminal regions of GPRI1 can function as a separable activation domain. GPRI1 and GPRI2 may function in some promoters in concert with a GBF through interaction with its Pro-rich region to enhance the transcriptional level of the corresponding genes.
Volume 43(1)
Pages 99-107
Published 2002-1
PMID 11828027
MeSH Amino Acid Sequence Arabidopsis / genetics* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Basic-Leucine Zipper Transcription Factors DNA, Complementary / genetics DNA, Complementary / isolation & purification DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism G-Box Binding Factors Molecular Sequence Data Protein Binding Protein Interaction Mapping Sequence Homology, Amino Acid Trans-Activators / genetics* Trans-Activators / metabolism Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic Two-Hybrid System Techniques
IF 4.059
Times Cited 23
Arabidopsis / Cultured plant cells, genes rpc00008