論文 - 詳細
| RRC ID | 2010 |
|---|---|
| 著者 | Nishiyama N, Iriyama A, Jang WD, Miyata K, Itaka K, Inoue Y, Takahashi H, Yanagi Y, Tamaki Y, Koyama H, Kataoka K. |
| タイトル | Light-induced gene transfer from packaged DNA enveloped in a dendrimeric photosensitizer. |
| ジャーナル | Nat Mater |
| Abstract |
The control of gene transfection in the body is a core issue in gene therapy. Photochemical internalization is a technology that allows light-induced delivery of DNA, drugs or other biological factors directly inside cells. Usually it requires that a photosensitizer be added to the drug-delivery system to photochemically destabilize the endosomal membrane. Here we present a system for in vivo DNA delivery in which these two components are assembled into one structure. This is a ternary complex composed of a core containing DNA packaged with cationic peptides and enveloped in the anionic dendrimer phthalocyanine, which provides the photosensitizing action. The ternary complex showed more than 100-fold photochemical enhancement of transgene expression in vitro with reduced photocytotoxicity. In an animal experiment, subconjuctival injection of the ternary complex followed by laser irradiation resulted in transgene expression only in the laser-irradiated site. This work demonstrates a new biomedical application for dendrimers, and the first success in the photochemical-internalization-mediated gene delivery in vivo. |
| 巻・号 | 4(12) |
| ページ | 934-41 |
| 公開日 | 2005-12-1 |
| DOI | 10.1038/nmat1524 |
| PII | nmat1524 |
| PMID | 16299510 |
| MeSH | Animals Conjunctiva / metabolism DNA / genetics* DNA / ultrastructure Dendrimers / chemistry* Endocytosis Flow Cytometry Fluoresceins / pharmacology Fluorescent Dyes / pharmacology Gene Transfer Techniques* Genes, Reporter HeLa Cells Humans Indoles / chemistry* Isoindoles Light Luciferases / metabolism Male Microscopy, Atomic Force Models, Biological Octanols / chemistry Photosensitizing Agents / chemistry* Rats Rats, Wistar Scattering, Radiation Static Electricity Transgenes Water / chemistry |
| IF | 38.663 |
| 引用数 | 270 |
| WOS 分野 | PHYSICS, APPLIED MATERIALS SCIENCE, MULTIDISCIPLINARY PHYSICS, CONDENSED MATTER CHEMISTRY, PHYSICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAcc-Luc+ (RDB02443) |