RRC ID 20116
Author Shibata M, Kashino Y, Satoh K, Koike H.
Title Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a glaucocystophyte, Cyanophora paradoxa.
Journal Plant Cell Physiol
Abstract Conditions for preparing oxygen-evolving thylakoid membranes and PSII complexes, and those for observing the PSII activity were investigated in a glaucocystophyte, Cyanophora paradoxa. The active thylakoid membranes were isolated either with a medium containing glycerol or with that containing high concentrations of sucrose, phosphate, and citrate. Active PSII particles were solubilized by octyl-beta-D-glucoside from thylakoid membranes and were separated by sucrose density gradient centrifugation. The thylakoid membranes and PSII particles showed an oxygen-evolving activity only in high-ionic-strength media. The extrinsic 33 kDa protein (PsbO) and the cytochrome c(550) (PsbV) were found to be present in the PSII particles as in cyanobacteria or red algae, but no 12 kDa protein (PsbU) was detected. The PsbO protein was classified as a land-plant type by its N-terminal amino acid sequence.
Volume 42(7)
Pages 733-41
Published 2001-7-1
DOI 10.1093/pcp/pce092
PMID 11479380
MeSH Absorptiometry, Photon Amino Acid Sequence Bacterial Proteins* Cell Fractionation Centrifugation, Density Gradient Citric Acid / pharmacology Cyanobacteria / metabolism Cytochrome c Group / chemistry Cytochrome c Group / isolation & purification Cytochrome c Group / metabolism* Eukaryota / chemistry Eukaryota / metabolism* Glucosides / pharmacology Glycerol / pharmacology Intracellular Membranes / metabolism* Molecular Sequence Data Oxygen / metabolism Phosphates / pharmacology Photosynthetic Reaction Center Complex Proteins / chemistry Photosynthetic Reaction Center Complex Proteins / isolation & purification Photosynthetic Reaction Center Complex Proteins / metabolism* Photosystem II Protein Complex* Rhodophyta / metabolism Sucrose / pharmacology Thylakoids / chemistry Thylakoids / metabolism*
IF 4.062
Times Cited 15
Algae NIES-547