論文 - 詳細
| RRC ID | 20317 |
|---|---|
| 著者 | Obata T, Shiraiwa Y. |
| タイトル | A novel eukaryotic selenoprotein in the haptophyte alga Emiliania huxleyi. |
| ジャーナル | J Biol Chem |
| Abstract |
The diversity of selenoproteins raises the question of why many life forms require selenium. Especially in photosynthetic organisms, the biochemical basis for the requirement for selenium is unclear because there is little information on selenoproteins. We found six selenium-containing proteins in a haptophyte alga, Emiliania huxleyi, which requires selenium for growth. The 27-kDa protein EhSEP2 was isolated, and its cDNA was cloned. The deduced amino acid sequence revealed that EhSEP2 is homologous to protein disulfide isomerase (PDI) and contains a highly conserved thioredoxin domain. The nucleotide sequence contains an in-frame TGA codon encoding selenocysteine at the position corresponding to the cysteine residue in the reaction center of known PDIs. However, no typical selenocysteine insertion sequence was found in the EhSEP2 cDNA. The EhSEP2 mRNA level was related to the abundance of selenium. E. huxleyi possesses a novel PDI-like selenoprotein and may have a novel type of selenocysteine insertion machinery. |
| 巻・号 | 280(18) |
| ページ | 18462-8 |
| 公開日 | 2005-5-6 |
| DOI | 10.1074/jbc.M501517200 |
| PII | S0021-9258(20)65799-4 |
| PMID | 15743763 |
| MeSH | Algal Proteins / chemistry* Algal Proteins / genetics* Amino Acid Sequence Base Sequence Eukaryota / chemistry Eukaryota / enzymology Eukaryota / genetics* Eukaryotic Cells / physiology* Molecular Sequence Data Protein Disulfide-Isomerases / chemistry Proteins / chemistry* Proteins / genetics* Selenium / physiology Selenoproteins Structural Homology, Protein |
| IF | 4.238 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| 藻類 | NIES-837 |