RRC ID 20369
著者 Yoshida M, Yoshida T, Takashima Y, Hosoda N, Hiroishi S.
タイトル Dynamics of microcystin-producing and non-microcystin-producing Microcystis populations is correlated with nitrate concentration in a Japanese lake.
ジャーナル FEMS Microbiol Lett
Abstract Temporal changes in hepatotoxin microcystin-producing and non-microcystin-producing Microcystis aeruginosa populations were examined in Lake Mikata, Japan. To monitor the densities of the total M. aeruginosa population and the potential microcystin-producing subpopulation, we used a quantitative real-time PCR assay targeting the phycocyanin intergenic spacer and the microcystin synthetase gene (mcyA), respectively. During the sampling period, the ratio of the mcyA subpopulation to the total M. aeruginosa varied considerably, from 0.5% to 35%. When surface nitrate concentrations increased, there was a rise in the relative abundance of the mcyA subpopulation. This was a positive correlation with the nitrate concentrations (r=0.53, P<0.05, n=14); whereas temperature and ortho-phosphate had no significant correlation with the presence of mcyA. Our data suggest that high nitrate loading may be a significant factor promoting the growth of the microcystin subpopulations within M. aeruginosa communities in Lake Mikata.
巻・号 266(1)
ページ 49-53
公開日 2007-1-1
DOI 10.1111/j.1574-6968.2006.00496.x
PII FML496
PMID 17092296
MeSH Bacterial Proteins / genetics DNA, Intergenic / chemistry Fresh Water / chemistry Fresh Water / microbiology* Genotype Japan Microcystins / biosynthesis* Microcystins / genetics Microcystis / classification Microcystis / metabolism* Nitrates / analysis* Peptide Synthases / genetics Phycocyanin / genetics Polymerase Chain Reaction Population Density Population Dynamics
IF 1.987
引用数 107
WOS 分野 MICROBIOLOGY
リソース情報
藻類 NIES-298