RRC ID 2061
Author Kanatsu-Shinohara M, Ogura A, Ikegawa M, Inoue K, Ogonuki N, Tashiro K, Toyokuni S, Honjo T, Shinohara T.
Title Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice.
Journal Proc Natl Acad Sci U S A
Abstract Sertoli cells play a pivotal role in spermatogenesis through their interactions with germ cells. To set up a strategy for treating male infertility caused by Sertoli cell dysfunction, we developed a Sertoli cell gene transfer system by using an adenovirus vector, which maintained long-term transgene expression in the testes of infertile mice. Introduction of an adenovirus carrying the mouse Steel (Sl) gene into Sertoli cells restored partial spermatogenesis in infertile Steel/Steel(dickie) (Sl/Sl(d)) mutant mouse testes. Although these males remained infertile, round spermatids and spermatozoa from the testes produced normal fertile offspring after intracytoplasmic injection into oocytes. None of the offspring showed evidence of germ line transmission of adenoviral DNA. Thus, we demonstrate a successful treatment for infertility by using a gene therapy vector. Therefore, adenovirus-mediated gene delivery into Sertoli cells not only provides an efficient and convenient means for studying germ cell-Sertoli cell interactions through manipulation of the germ cell microenvironment in vivo, but also is a useful method to treat male infertility resulting from a Sertoli cell defect.
Volume 99(3)
Pages 1383-8
Published 2002-2-5
DOI 10.1073/pnas.022646399
PII 022646399
PMID 11818536
PMC PMC122199
MeSH Adenoviridae / genetics* Animals Chromatin / ultrastructure Crosses, Genetic Gene Transfer Techniques* Genetic Vectors* Infertility, Male / genetics* Insemination / genetics Male Mice Mice, Inbred C57BL Mice, Inbred DBA Microinjections Spermatids / cytology Spermatogenesis Spermatozoa / cytology beta-Galactosidase / genetics
IF 9.412
Times Cited 55
DNA material AxCALacZ (RDB1745)