Reference - Detail
| RRC ID | 20748 |
|---|---|
| Author | Toda H, Yazawa M, Yagi K. |
| Title | Amino acid sequence of calmodulin from Euglena gracilis. |
| Journal | Eur J Biochem |
| Abstract |
The complete amino acid sequence of calmodulin from Euglena gracilis was determined by isolation and sequence analyses of peptides derived from calmodulin by digestion with trypsin and Staphylococcus aureus V8 protease. Euglena calmodulin consists of 148 amino acid residues; it lacks tryptophan and cysteine and contains one tyrosine, three histidine and two NE-trimethyllysine residues/molecule of the protein. Its N-terminus was blocked with an acetyl group and C-terminal lysine was trimethylated. Euglena calmodulin is the first calmodulin so far examined in which the C-terminal lysine is trimethylated. The comparison of amino acid sequences between Euglena and human brain calmodulins indicated 17 amino acid substitutions in Euglena calmodulin. |
| Volume | 205(2) |
| Pages | 653-60 |
| Published | 1992-4-15 |
| DOI | 10.1111/j.1432-1033.1992.tb16824.x |
| PMID | 1572365 |
| MeSH | Amino Acid Sequence Animals Calmodulin / chemistry* Calmodulin / genetics Calmodulin / isolation & purification Chromatography, High Pressure Liquid Euglena gracilis / genetics Euglena gracilis / metabolism* Humans Molecular Sequence Data Peptide Fragments / isolation & purification Sequence Homology, Nucleic Acid Serine Endopeptidases Trypsin |
| Times Cited | 16 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Algae | NIES-48 |