論文 - 詳細
| RRC ID | 20790 |
|---|---|
| 著者 | Miura K, Yamano T, Yoshioka S, Kohinata T, Inoue Y, Taniguchi F, Asamizu E, Nakamura Y, Tabata S, Yamato KT, Ohyama K, Fukuzawa H. |
| タイトル | Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. |
| ジャーナル | Plant Physiol |
| Abstract |
Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO2 whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM. |
| 巻・号 | 135(3) |
| ページ | 1595-607 |
| 公開日 | 2004-7-1 |
| DOI | 10.1104/pp.104.041400 |
| PII | pp.104.041400 |
| PMID | 15235119 |
| PMC | PMC519074 |
| MeSH | Acclimatization Amino Acid Sequence Animals Carbon / metabolism* Carbon Dioxide / metabolism Carbon Dioxide / pharmacology* Chlamydomonas reinhardtii / drug effects Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / metabolism* Conserved Sequence Gene Expression Profiling / methods* Gene Expression Regulation / genetics Humans Molecular Sequence Data Protozoan Proteins / chemistry Protozoan Proteins / genetics Sequence Alignment Sequence Homology, Amino Acid |
| IF | 6.902 |
| 引用数 | 133 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2235 |