| RRC ID |
20792
|
| 著者 |
Abe J, Kubo T, Takagi Y, Saito T, Miura K, Fukuzawa H, Matsuda Y.
|
| タイトル |
The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii.
|
| ジャーナル |
Curr Genet
|
| Abstract |
Cells of Chlamydomonas reinhardtii undergo gametogenesis to produce sexually competent gametes under nitrogen-starved conditions. By using a synchronized system for gametogenesis of early G1 cells, several previously identified marker genes and 18 novel nitrogen-starved gametogenesis (NSG) genes isolated by macroarray analysis were placed into at least three temporal classes of expression. Early genes are induced transiently in the first 2 h after transfer to nitrogen-free medium. Middle genes are strongly induced between 3 h and 4 h after nitrogen removal, a time corresponding to the acquisition of mating competency, suggesting their involvement in the gamete program. Late genes are induced between 5 h and 8 h after nitrogen removal, a time after the completion of gametic differentiation, suggesting that they are not directly involved in the formation of sexually competent gametes. All of the 18 NSG genes examined are induced in both mating-type plus and minus gametes and about two-thirds of the genes are also expressed in the mitotic cell cycle, especially at S/M phases.
|
| 巻・号 |
46(5)
|
| ページ |
304-15
|
| 公開日 |
2004-11-1
|
| DOI |
10.1007/s00294-004-0526-4
|
| PMID |
15459796
|
| MeSH |
Animals
Blotting, Northern
Cell Differentiation
Cell Division / genetics*
Chlamydomonas reinhardtii / growth & development
Chlamydomonas reinhardtii / physiology*
Culture Media
G1 Phase / genetics*
Gametogenesis*
Gene Expression Profiling*
Germ Cells / physiology
Nitrogen / deficiency
S Phase / genetics*
|
| IF |
3.464
|
| 引用数 |
38
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 藻類 |
NIES-2235 |
