RRC ID 20833
著者 Issarapayup K, Powtongsook S, Pavasant P.
タイトル Flat panel airlift photobioreactors for cultivation of vegetative cells of microalga Haematococcus pluvialis.
ジャーナル J Biotechnol
Abstract The novel flat panel airlift photobioreactor (FP-ALPBR) was proposed as an alternative system for the cultivation of Haematococcus pluvialis NIES-144. Changes in the efficiency of the system were tested in response to variations in two engineering parameters: the ratio between the downcomer and riser cross-sectional areas (A(d)/A(r)) and the size of the system (as determined by the length of the panel) and to one operating parameter: the superficial gas velocity (u(sg)). The best growth performance was obtained by operating the system at a superficial velocity of 0.4 cms(-1) and with a downcomer-to-riser cross-sectional area ratio of 0.4. The 17-l FP-ALPBR system was capable of giving reasonable growth characteristics with a maximum cell density of 4.1 x 10(5)cell ml(-1) and specific growth rate of 0.52 day(-1) being achieved. A similar level of performance was obtained from the 90-l FP-ALPBR system, i.e., cell density=40 x 10(4)cell ml(-1) but with a slight decrease in specific growth rate to 0.39 day(-1). The performances of these two differently sized FP-ALPBRs were compared with two conventional cylindrical airlift photobioreactors (C-ALPBRs) of different dimensions. The 90-l FP-ALPBR exhibited reasonably good performance when compared with the two 17-l systems (both C- and FP-ALPBRs); however, the best growth rate was observed using the 3-l C-ALPBR. Semi-continuous cultures, which could be periodically harvested at a reasonably high growth rate, were successfully created. Of all the systems investigated in this study, the 90-l FP-ALPBR was found to be the most cost-effective, as it could cultivate 18 g of alga for approximately US$ 21.
巻・号 142(3-4)
ページ 227-32
公開日 2009-7-15
DOI 10.1016/j.jbiotec.2009.04.014
PII S0168-1656(09)00186-2
PMID 19422861
MeSH Bioreactors* Cell Count Cell Culture Techniques / instrumentation* Cell Culture Techniques / methods Chlorophyta / cytology Chlorophyta / growth & development* Equipment Design* Gases Photochemical Processes Xanthophylls
IF 3.503
引用数 37
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
藻類 NIES-144