RRC ID 20878
Author Stern RF, Horak A, Andrew RL, Coffroth MA, Andersen RA, Küpper FC, Jameson I, Hoppenrath M, Véron B, Kasai F, Brand J, James ER, Keeling PJ.
Title Environmental barcoding reveals massive dinoflagellate diversity in marine environments.
Journal PLoS ONE
Abstract BACKGROUND:Dinoflagellates are an ecologically important group of protists with important functions as primary producers, coral symbionts and in toxic red tides. Although widely studied, the natural diversity of dinoflagellates is not well known. DNA barcoding has been utilized successfully for many protist groups. We used this approach to systematically sample known "species", as a reference to measure the natural diversity in three marine environments.
METHODOLOGY/PRINCIPAL FINDINGS:In this study, we assembled a large cytochrome c oxidase 1 (COI) barcode database from 8 public algal culture collections plus 3 private collections worldwide resulting in 336 individual barcodes linked to specific cultures. We demonstrate that COI can identify to the species level in 15 dinoflagellate genera, generally in agreement with existing species names. Exceptions were found in species belonging to genera that were generally already known to be taxonomically challenging, such as Alexandrium or Symbiodinium. Using this barcode database as a baseline for cultured dinoflagellate diversity, we investigated the natural diversity in three diverse marine environments (Northeast Pacific, Northwest Atlantic, and Caribbean), including an evaluation of single-cell barcoding to identify uncultivated groups. From all three environments, the great majority of barcodes were not represented by any known cultured dinoflagellate, and we also observed an explosion in the diversity of genera that previously contained a modest number of known species, belonging to Kareniaceae. In total, 91.5% of non-identical environmental barcodes represent distinct species, but only 51 out of 603 unique environmental barcodes could be linked to cultured species using a conservative cut-off based on distances between cultured species.
CONCLUSIONS/SIGNIFICANCE:COI barcoding was successful in identifying species from 70% of cultured genera. When applied to environmental samples, it revealed a massive amount of natural diversity in dinoflagellates. This highlights the extent to which we underestimate microbial diversity in the environment.
Volume 5(11)
Pages e13991
Published 2010-11-15
DOI 10.1371/journal.pone.0013991
PMID 21085582
PMC PMC2981561
MeSH Animals Atlantic Ocean Biodiversity* Caribbean Region Cluster Analysis DNA, Mitochondrial / chemistry DNA, Mitochondrial / genetics Databases, Nucleic Acid Dinoflagellida / classification Dinoflagellida / genetics* Dinoflagellida / growth & development* Electron Transport Complex IV / genetics Genetic Variation* Molecular Sequence Data Pacific Ocean Phylogeny Protozoan Proteins / genetics Seawater / microbiology Sequence Analysis, DNA Species Specificity
IF 2.776
Times Cited 54
Algae NIES-1367