| RRC ID |
20914
|
| Author |
Ohnuma M, Kuroiwa T, Tanaka K.
|
| Title |
Optimization of cryopreservation conditions for the unicellular red alga Cyanidioschyzon merolae.
|
| Journal |
J Gen Appl Microbiol
|
| Abstract |
Cryopreservation is essential for maintaining stable stocks of organisms. We report the development of a method for cryopreservation of the unicellular red alga Cyanidioschyzon merolae, a model organism for the investigation of the basic architecture of photosynthetic eukaryotes. Glycerol, dimethyl sulfoxide and methanol were examined for their ability to protect the cell from cryoinjury and/or cytotoxicity. It was found that methanol was the most effective as a cryoprotectant for C. merolae. After the optimized setting of parameters such as working concentration of cryoprotectant and the period of slow cooling, cultures were supplemented with 5% (v/v) methanol and frozen by slow cooling using a passive-freezing unit, followed by plunging into liquid nitrogen. We found C. merolae cells retained greater than 80% viability for at least 83 days in storage.
|
| Volume |
57(3)
|
| Pages |
137-43
|
| Published |
2011-1-1
|
| DOI |
10.2323/jgam.57.137
|
| PII |
JST.JSTAGE/jgam/57.137
|
| PMID |
21817825
|
| MeSH |
Cryopreservation / methods*
Cryoprotective Agents / metabolism
Dimethyl Sulfoxide / metabolism
Freezing
Glycerol / metabolism
Methanol / metabolism
Rhodophyta / radiation effects*
Time Factors
|
| IF |
1.442
|
| Times Cited |
4
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| Algae |
NIES-1332 |
