論文 - 詳細
| RRC ID | 20915 |
|---|---|
| 著者 | Nakazawa A, Nishii I. |
| タイトル | Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. |
| ジャーナル | Cryo Letters |
| Abstract |
A number of volvocalean green algae species were subjected to a two-step cryopreservation protocol with various cryoprotectants. Potential cryoprotectants were methanol (DMSO), N,N-dimethylformamide (DMF), N,N-dimethylacetamide, N-methylformamide, and hydroxyacetone (HA). We confirmed prior reports that MeOH was effective for cryopreserving Chlamydomonas, but did not work well for larger volvocaleans such as Volvox. In contrast, DMF and HA were effective for both unicellular and multicellular representatives. When we used a cold-inducible transposon to probe Southern blots of Volvox DNA samples taken before and after storage for one month in LN, we could detect no differences, indicating that the genome had remained relatively stable and that the transposon had not been induced by the cryopreservation procedure. We believe these methods will facilitate long-term storage of several volvocine algal species, including Volvox strains harboring transposon-induced mutations of developmental interest. |
| 巻・号 | 33(3) |
| ページ | 202-13 |
| 公開日 | 2012-1-1 |
| PMID | 22825787 |
| MeSH | Acetone / analogs & derivatives* Acetone / chemistry Chlamydomonas / cytology Chlorophyta / cytology* Cryopreservation / methods* Cryoprotective Agents* / chemistry Formamides* / chemistry Volvox / cytology |
| IF | 0.702 |
| 引用数 | 4 |
| WOS 分野 | BIOLOGY PHYSIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 藻類 | NIES-418 NIES-468 NIES-545 NIES-564 NIES-574 NIES-717 NIES-721 NIES-728 NIES-729 NIES-730 NIES-731 NIES-877 NIES-1331 NIES-1361 NIES-1710 |