RRC ID 2094
著者 Kato J, Hashimoto M.
タイトル Construction of consecutive deletions of the Escherichia coli chromosome.
ジャーナル Mol Syst Biol
Abstract The minimal set of genetic information necessary and sufficient to sustain a functioning cell contains not only trans-acting genes, but also cis-acting chromosomal regions that cannot be complemented by plasmids carrying these regions. In Escherichia coli (E. coli), only one chromosomal region, the origin of replication has been identified to be cis-acting. We constructed a series of mutants with long-range deletions, and the chromosomal regions containing trans-acting essential genes were deleted in the presence of plasmids complementing the deleted genes. The deleted regions cover all regions of the chromosome except for the origin and terminus of replication. The terminus affects cell growth, but is not essential. Our results indicate that the origin of DNA replication is the only vital, unique cis-acting DNA sequence in the E. coli chromosome necessary for survival.
巻・号 3
ページ 132
公開日 2007-1-1
DOI 10.1038/msb4100174
PII msb4100174
PMID 17700540
PMC PMC1964801
MeSH Chromosomes, Bacterial / genetics* DNA Replication / genetics DNA, Bacterial / genetics Escherichia coli / genetics* Gene Expression Regulation, Bacterial Genes, Bacterial* Genetic Complementation Test Plasmids / genetics Replication Origin / genetics Sequence Deletion*
IF 8.991
引用数 93
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
情報 E.coli