Reference - Detail
|Author||Onami H, Nagai N, Machida S, Kumasaka N, Wakusawa R, Ishikawa Y, Sonoda H, Sato Y, Abe T.|
|Title||Reduction of laser-induced choroidal neovascularization by intravitreal vasohibin-1 in monkey eyes.|
|Journal||Retina (Philadelphia, Pa.)|
PURPOSE:To determine whether intravitreal vasohibin-1 will reduce the grade of the choroidal neovascularization in monkey eyes.
METHODS:Choroidal neovascularizations were induced in 12 monkey eyes by laser photocoagulation. Three monkeys were evaluated for the safety of the vasohibin-1 injections, 6 monkeys for the effects of a single injection, and 3 monkeys for repeated injections of vasohibin-1. Ophthalmoscopy, fluorescein angiography, focal electroretinograms, and optical coherence tomography were used for the evaluations. The level of vascular endothelial growth factor in the aqueous was determined by enzyme-linked immunosorbent assay. Immunohistochemistry was performed.
RESULTS:An intravitreal injection of 10 μg of vasohibin-1 induced mild intraocular inflammation. Eyes with an intravitreal injection of 0.1 μg and 1.0 μg of vasohibin-1 had significant less fluorescein leakage from the choroidal neovascularizations and larger amplitude focal electroretinograms than that of vehicle-injected eyes. Similar results were obtained by repeated injections of 0.1 μg of vasohibin-1. Immunohistochemistry showed that vasohibin-1 was expressed mainly in the endothelial cells within the choroidal neovascularizations. The vascular endothelial growth factor level was not significantly altered by intravitreal vasohibin-1.
CONCLUSION:The reduction of the laser-induced choroidal neovascularizations and preservation of macular function in monkey by intravitreal vasohibin-1 suggest that it should be considered for suppressing choroidal neovascularizations in humans.
|MeSH||Angiogenesis Inhibitors / administration & dosage* Angiogenesis Inhibitors / metabolism Animals Cell Cycle Proteins / administration & dosage* Cell Cycle Proteins / metabolism Choroidal Neovascularization / drug therapy* Choroidal Neovascularization / metabolism Choroidal Neovascularization / pathology Choroidal Neovascularization / physiopathology Disease Models, Animal Electroretinography / drug effects Enzyme-Linked Immunosorbent Assay Fluorescein Angiography Immunohistochemistry Intravitreal Injections Macaca Ophthalmoscopy Tomography, Optical Coherence Vascular Endothelial Growth Factor A / metabolism|