RRC ID 20963
Author Kim HL, Park MB, Park YS.
Title Tetrahydrobiopterin is functionally distinguishable from tetrahydrodictyopterin in Dictyostelium discoideum Ax2.
Journal FEBS Lett
Abstract Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that dihydropteridine reductase (DHPR) and dihydrofolate reductase (DHFR) constitute the regeneration pathway of both BH4 and DH4, the results suggested that BH4 is a preferential cofactor for PAH in vivo, not a secondary product of DH4, which functions mainly as an antioxidant. Our result also demonstrated that PAH may be essential for Dictyostelium growth in nature, and thus it appears that the organism has evolved a strategy to maintain BH4 level via regeneration pathway at the expense of DH4 under oxidative stress conditions.
Volume 585(19)
Pages 3047-51
Published 2011-10-3
DOI 10.1016/j.febslet.2011.08.026
PII S0014-5793(11)00628-4
PMID 21871890
MeSH Biopterin / analogs & derivatives* Biopterin / chemistry Biopterin / metabolism Dictyostelium / chemistry Dictyostelium / genetics Dictyostelium / metabolism* Dihydropteridine Reductase / genetics Dihydropteridine Reductase / metabolism Gene Knockdown Techniques Oxidative Stress Protozoan Proteins / genetics Protozoan Proteins / metabolism Stereoisomerism Tetrahydrofolate Dehydrogenase / genetics Tetrahydrofolate Dehydrogenase / metabolism
IF 3.057
Times Cited 4
Cellular slime molds S00001