| RRC ID |
20
|
| 著者 |
Kajstura J, Rota M, Whang B, Cascapera S, Hosoda T, Bearzi C, Nurzynska D, Kasahara H, Zias E, Bonafé M, Nadal-Ginard B, Torella D, Nascimbene A, Quaini F, Urbanek K, Leri A, Anversa P.
|
| タイトル |
Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion.
|
| ジャーナル |
Circ Res
|
| Abstract |
Recent studies in mice have challenged the ability of bone marrow cells (BMCs) to differentiate into myocytes and coronary vessels. The claim has also been made that BMCs acquire a cell phenotype different from the blood lineages only by fusing with resident cells. Technical problems exist in the induction of myocardial infarction and the successful injection of BMCs in the mouse heart. Similarly, the accurate analysis of the cell populations implicated in the regeneration of the dead tissue is complex and these factors together may account for the negative findings. In this study, we have implemented a simple protocol that can easily be reproduced and have reevaluated whether injection of BMCs restores the infarcted myocardium in mice and whether cell fusion is involved in tissue reconstitution. For this purpose, c-kit-positive BMCs were obtained from male transgenic mice expressing enhanced green fluorescence protein (EGFP). EGFP and the Y-chromosome were used as markers of the progeny of the transplanted cells in the recipient heart. By this approach, we have demonstrated that BMCs, when properly administrated in the infarcted heart, efficiently differentiate into myocytes and coronary vessels with no detectable differentiation into hemopoietic lineages. However, BMCs have no apparent paracrine effect on the growth behavior of the surviving myocardium. Within the infarct, in 10 days, nearly 4.5 million biochemically and morphologically differentiated myocytes together with coronary arterioles and capillary structures were generated independently of cell fusion. In conclusion, BMCs adopt the cardiac cell lineages and have an important therapeutic impact on ischemic heart failure.
|
| 巻・号 |
96(1)
|
| ページ |
127-37
|
| 公開日 |
2005-1-7
|
| DOI |
10.1161/01.RES.0000151843.79801.60
|
| PII |
01.RES.0000151843.79801.60
|
| PMID |
15569828
|
| MeSH |
Animals
Arterioles / cytology
Artifacts
Bone Marrow Cells / cytology*
Capillaries / cytology
Cell Differentiation
Cell Fusion
Cell Lineage*
Endothelial Cells / cytology
Female
Genes, Reporter
Graft Survival
Green Fluorescent Proteins / analysis
Heart / physiology
Hematopoietic Stem Cell Transplantation
Humans
Injections, Intralesional
Male
Mice
Mice, Transgenic
Myocardial Contraction
Myocardial Infarction / surgery*
Myocytes, Cardiac / cytology
Myocytes, Smooth Muscle / cytology
Organ Specificity
Paracrine Communication
Proto-Oncogene Proteins c-kit / analysis
Regeneration
Stem Cell Transplantation*
Ventricular Function, Left
Y Chromosome
|
| IF |
14.467
|
| 引用数 |
373
|
|
WOS 分野
|
PERIPHERAL VASCULAR DISEASE
HEMATOLOGY
CARDIAC & CARDIOVASCULAR SYSTEMS
|
| リソース情報 |
| 実験動物マウス |
RBRC01198 |
