RRC ID 21148
Author Neumetzler L, Humphrey T, Lumba S, Snyder S, Yeats TH, Usadel B, Vasilevski A, Patel J, Rose JK, Persson S, Bonetta D.
Title The FRIABLE1 gene product affects cell adhesion in Arabidopsis.
Journal PLoS One
Abstract Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion.
Volume 7(8)
Pages e42914
Published 2012-1-1
DOI 10.1371/journal.pone.0042914
PII PONE-D-11-14710
PMID 22916179
PMC PMC3419242
MeSH Arabidopsis / cytology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology* Arabinose / metabolism Cell Adhesion / genetics Cell Adhesion / physiology* Cloning, Molecular Galactose / metabolism Golgi Apparatus / metabolism Pectins / metabolism
IF 2.74
Times Cited 27
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pdx34088 pdx49027 pdz65499 pda12966 pda00129 pda12120 pda13381