RRC ID 21268
著者 Unno A, Kachi S, Batanova TA, Ohno T, Elhawary N, Kitoh K, Takashima Y.
タイトル Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver.
ジャーナル Exp Parasitol
Abstract The intracellular parasite Toxoplasma gondii is thought to disseminate throughout the host by circulation of tachyzoite-infected leukocytes in the blood, and adherence and migration of such leukocytes into solid tissues. However, it is unclear whether T. gondii-infected leukocytes can migrate to solid organs via the general circulation. In this study, we developed a real-time quantitative PCR (qRT-PCR) method to determine the rate of infection of peripheral blood mononuclear cells (PBMCs) flowing into and remaining within solid organs in mice. A transgenic T. gondii parasite line derived from the PLK strain that expresses DsRed Express, and transgenic green fluorescent protein-positive PBMCs, were used for these experiments. Tachyzoite-infected PBMCs were injected into mouse tail veins and qRT-PCR was used to measure the infection rates of the PBMCs remaining in the lungs, liver, spleen and brain. We found that the PBMCs in the lungs and liver had statistically higher infection rates than that of the original inoculum; this difference was statistically significant. However, the PBMC infection rate in the spleen showed no such enhancement. These results show that tachyzoite-infected PBMCs in the general circulation remain in the lungs and liver more effectively than non-infected PBMCs.
巻・号 134(2)
ページ 160-4
公開日 2013-6-1
DOI 10.1016/j.exppara.2013.03.006
PII S0014-4894(13)00076-3
PMID 23538031
MeSH Animals Chlorocebus aethiops DNA, Protozoan / isolation & purification Green Fluorescent Proteins Heart Ventricles / parasitology Heart Ventricles / pathology Leukocytes, Mononuclear / cytology Leukocytes, Mononuclear / parasitology* Liver / parasitology Liver / pathology* Luminescent Agents Lung / parasitology Lung / pathology* Male Mice Mice, Inbred C57BL Real-Time Polymerase Chain Reaction Spleen / parasitology Spleen / pathology Toxoplasma / genetics Toxoplasma / isolation & purification Toxoplasma / physiology* Toxoplasmosis, Animal / blood Toxoplasmosis, Animal / parasitology Toxoplasmosis, Animal / pathology* Vero Cells
IF 1.69
引用数 12
WOS 分野 PARASITOLOGY
リソース情報
実験動物マウス RBRC00267