Reference - Detail
| RRC ID | 21397 |
|---|---|
| Author | Tsuda T, Suzuki T, Kojima S. |
| Title | Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase. |
| Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| Abstract |
Bacillus subtilis YwfE, an L-amino-acid ligase, catalyzes the formation of an α-dipeptide from L-amino acids in an ATP-dependent manner. In order to elucidate the substrate-recognition mode and the reaction mechanism of this ligase, native and selenomethionine-derivatized (SeMet) crystals of YwfE in the presence of ADP, MgCl(2) and the dipeptide L-Ala-L-Gln were obtained using the hanging-drop vapour-diffusion method. These crystals diffracted to 1.9 and 2.8 Å resolution, respectively. Preliminary SAD phase calculations using the data set from the SeMet crystal suggested that the crystal belonged to the hexagonal space group P6(5)22, with unit-cell parameters a = b = 90.85, c = 250.31 Å, and contained one molecule in the asymmetric unit with a solvent content of 57.3%. |
| Volume | 68(Pt 2) |
| Pages | 203-6 |
| Published | 2012-2-1 |
| DOI | 10.1107/S174430911105425X |
| PII | S174430911105425X |
| PMID | 22298000 |
| PMC | PMC3274404 |
| MeSH | Bacillus subtilis / enzymology* Crystallization Crystallography, X-Ray Peptide Synthases / chemistry* |
| IF | 0.968 |
| Times Cited | 3 |
| WOS Category | CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 1465 |