| RRC ID |
21397
|
| Author |
Tsuda T, Suzuki T, Kojima S.
|
| Title |
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
|
| Journal |
Acta Crystallogr Sect F Struct Biol Cryst Commun
|
| Abstract |
Bacillus subtilis YwfE, an L-amino-acid ligase, catalyzes the formation of an α-dipeptide from L-amino acids in an ATP-dependent manner. In order to elucidate the substrate-recognition mode and the reaction mechanism of this ligase, native and selenomethionine-derivatized (SeMet) crystals of YwfE in the presence of ADP, MgCl(2) and the dipeptide L-Ala-L-Gln were obtained using the hanging-drop vapour-diffusion method. These crystals diffracted to 1.9 and 2.8 Å resolution, respectively. Preliminary SAD phase calculations using the data set from the SeMet crystal suggested that the crystal belonged to the hexagonal space group P6(5)22, with unit-cell parameters a = b = 90.85, c = 250.31 Å, and contained one molecule in the asymmetric unit with a solvent content of 57.3%.
|
| Volume |
68(Pt 2)
|
| Pages |
203-6
|
| Published |
2012-2-1
|
| DOI |
10.1107/S174430911105425X
|
| PII |
S174430911105425X
|
| PMID |
22298000
|
| PMC |
PMC3274404
|
| MeSH |
Bacillus subtilis / enzymology*
Crystallization
Crystallography, X-Ray
Peptide Synthases / chemistry*
|
| IF |
0.968
|
| Times Cited |
3
|
|
WOS Category
|
CRYSTALLOGRAPHY
BIOPHYSICS
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 1465 |
