論文 - 詳細
| RRC ID | 21411 |
|---|---|
| 著者 | Hotta Y, Sato H, Hosoda A, Tamura H. |
| タイトル | MALDI-TOF MS analysis of ribosomal proteins coded in S10 and spc operons rapidly classified the Sphingomonadaceae as alkylphenol polyethoxylate-degrading bacteria from the environment. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using ribosomal subunit proteins coded in the S10-spc-alpha operon as biomarkers was applied for the classification of the Sphingomonadaceae from the environment. To construct a ribosomal protein database, S10-spc-alpha operon of type strains of the Sphingomonadaceae and their related alkylphenol polyethoxylate (APEO(n) )-degrading bacteria were sequenced using specific primers designed based on nucleotide sequences of genome-sequenced strains. The observed MALDI mass spectra of intact cells were compared with the theoretical mass of the constructed ribosomal protein database. The nine selected biomarkers coded in the S10-spc-alpha operon, L18, L22, L24, L29, L30, S08, S14, S17, and S19, could successfully distinguish the Sphingopyxis terrae NBRC 15098(T) and APEO(n) -degrading bacteria strain BSN20, despite only one base difference in the 16S rRNA gene sequence. This method, named the S10-GERMS (S10-spc-alpha operon gene-encoded ribosomal protein mass spectrum) method, is a significantly useful tool for bacterial discrimination of the Sphingomonadaceae at the strain level and can detect and monitor the main APEO(n) -degrading bacteria in the environment. |
| 巻・号 | 330(1) |
| ページ | 23-9 |
| 公開日 | 2012-5-1 |
| DOI | 10.1111/j.1574-6968.2012.02525.x |
| PMID | 22324315 |
| MeSH | Bacterial Proteins / analysis Bacterial Proteins / genetics Bacteriological Techniques / methods* DNA, Bacterial / chemistry DNA, Bacterial / genetics Environmental Microbiology Molecular Sequence Data Polyethylene Glycols / metabolism* Ribosomal Proteins / analysis* Ribosomal Proteins / genetics Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Sphingomonadaceae / chemistry* Sphingomonadaceae / classification Sphingomonadaceae / isolation & purification* Sphingomonadaceae / metabolism |
| IF | 1.987 |
| 引用数 | 9 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 11457 JCM 14161 JCM14844 JCM15910 |