RRC ID 21411
著者 Hotta Y, Sato H, Hosoda A, Tamura H.
タイトル MALDI-TOF MS analysis of ribosomal proteins coded in S10 and spc operons rapidly classified the Sphingomonadaceae as alkylphenol polyethoxylate-degrading bacteria from the environment.
ジャーナル FEMS Microbiol Lett
Abstract Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using ribosomal subunit proteins coded in the S10-spc-alpha operon as biomarkers was applied for the classification of the Sphingomonadaceae from the environment. To construct a ribosomal protein database, S10-spc-alpha operon of type strains of the Sphingomonadaceae and their related alkylphenol polyethoxylate (APEO(n) )-degrading bacteria were sequenced using specific primers designed based on nucleotide sequences of genome-sequenced strains. The observed MALDI mass spectra of intact cells were compared with the theoretical mass of the constructed ribosomal protein database. The nine selected biomarkers coded in the S10-spc-alpha operon, L18, L22, L24, L29, L30, S08, S14, S17, and S19, could successfully distinguish the Sphingopyxis terrae NBRC 15098(T) and APEO(n) -degrading bacteria strain BSN20, despite only one base difference in the 16S rRNA gene sequence. This method, named the S10-GERMS (S10-spc-alpha operon gene-encoded ribosomal protein mass spectrum) method, is a significantly useful tool for bacterial discrimination of the Sphingomonadaceae at the strain level and can detect and monitor the main APEO(n) -degrading bacteria in the environment.
巻・号 330(1)
ページ 23-9
公開日 2012-5-1
DOI 10.1111/j.1574-6968.2012.02525.x
PMID 22324315
MeSH Bacterial Proteins / analysis Bacterial Proteins / genetics Bacteriological Techniques / methods* DNA, Bacterial / chemistry DNA, Bacterial / genetics Environmental Microbiology Molecular Sequence Data Polyethylene Glycols / metabolism* Ribosomal Proteins / analysis* Ribosomal Proteins / genetics Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Sphingomonadaceae / chemistry* Sphingomonadaceae / classification Sphingomonadaceae / isolation & purification* Sphingomonadaceae / metabolism
IF 1.987
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 11457 JCM 14161 JCM14844 JCM15910