| RRC ID |
21431
|
| 著者 |
Hayashi E, Mokudai T, Yamada Y, Nakamura K, Kanno T, Sasaki K, Niwano Y.
|
| タイトル |
In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
The present study aimed to evaluate in vitro and in vivo antibacterial activity of hydroxyl radical generation system by photolysis of H(2)O(2), which is a new disinfection system for the treatment of oral infection diseases such as periodontitis developed in our laboratory. Firstly, generation of the hydroxyl radical by the photolysis of H(2)O(2) in which 1 mol l(-1) H(2)O(2) was irradiated with a dual wavelength-light emitting diode (LED) at wavelengths of 400 and 465 nm was confirmed by applying an electron spin resonance-spin trapping technique. Secondly, the bactericidal effect of the system was examined under a similar condition in which Staphylococcus aureus suspended in 1 mol l(-1) H(2)O(2) was irradiated with LED light, resulting in substantial reduction of the colony forming unit (CFU) of the bacteria within a short time as 2 min. Finally, in vivo antibacterial effect of the photolysis of H(2)O(2) on a rat model of S. aureus infection was evaluated by a culture study. Since a significant reduction of recovered CFU of S. aureus was obtained, it is expected that in vitro antibacterial effect attributable to hydroxyl radicals generated by photolysis of H(2)O(2) could be well reflected in in vivo superficial bacterial infection.
|
| 巻・号 |
114(2)
|
| ページ |
193-7
|
| 公開日 |
2012-8-1
|
| DOI |
10.1016/j.jbiosc.2012.03.020
|
| PII |
S1389-1723(12)00156-9
|
| PMID |
22578595
|
| MeSH |
Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Colony Count, Microbial
Disinfection / methods*
Electron Spin Resonance Spectroscopy
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / radiation effects*
Hydroxyl Radical / chemistry
Hydroxyl Radical / pharmacology*
Male
Photolysis / radiation effects*
Rats
Rats, Wistar
Staphylococcal Infections / microbiology
Staphylococcal Infections / therapy
Staphylococcus aureus / drug effects*
|
| IF |
2.366
|
| 引用数 |
19
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 2413 |
