RRC ID 21431
Author Hayashi E, Mokudai T, Yamada Y, Nakamura K, Kanno T, Sasaki K, Niwano Y.
Title In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide.
Journal J. Biosci. Bioeng.
Abstract The present study aimed to evaluate in vitro and in vivo antibacterial activity of hydroxyl radical generation system by photolysis of H(2)O(2), which is a new disinfection system for the treatment of oral infection diseases such as periodontitis developed in our laboratory. Firstly, generation of the hydroxyl radical by the photolysis of H(2)O(2) in which 1 mol l(-1) H(2)O(2) was irradiated with a dual wavelength-light emitting diode (LED) at wavelengths of 400 and 465 nm was confirmed by applying an electron spin resonance-spin trapping technique. Secondly, the bactericidal effect of the system was examined under a similar condition in which Staphylococcus aureus suspended in 1 mol l(-1) H(2)O(2) was irradiated with LED light, resulting in substantial reduction of the colony forming unit (CFU) of the bacteria within a short time as 2 min. Finally, in vivo antibacterial effect of the photolysis of H(2)O(2) on a rat model of S. aureus infection was evaluated by a culture study. Since a significant reduction of recovered CFU of S. aureus was obtained, it is expected that in vitro antibacterial effect attributable to hydroxyl radicals generated by photolysis of H(2)O(2) could be well reflected in in vivo superficial bacterial infection.
Volume 114(2)
Pages 193-7
Published 2012-8
DOI 10.1016/j.jbiosc.2012.03.020
PII S1389-1723(12)00156-9
PMID 22578595
MeSH Animals Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology* Colony Count, Microbial Disinfection / methods* Electron Spin Resonance Spectroscopy Hydrogen Peroxide / chemistry Hydrogen Peroxide / radiation effects* Hydroxyl Radical / chemistry Hydroxyl Radical / pharmacology* Male Photolysis / radiation effects* Rats Rats, Wistar Staphylococcal Infections / microbiology Staphylococcal Infections / therapy Staphylococcus aureus / drug effects*
IF 2.015
Times Cited 13
General Microbes JCM 2413