RRC ID 21470
著者 Li X, Liu H, Xu Y, Xu F, Wang L, You J, Li S, Jin L.
タイトル Chicken egg yolk antibody (IgY) controls Solobacterium moorei under in vitro and in vivo conditions.
ジャーナル Appl Biochem Biotechnol
Abstract Solobacterium moorei is a causative agent in diseases such as oral halitosis, bacteremia, and necrobacillosis-associated thrombophlebitis. The objective of this study was to determine the effectiveness of chicken egg yolk antibody (IgY) in controlling S. moorei. Intact S. moorei cells were used as an immunogen to immunize four White Leghorn laying hens. IgY, extracted from egg yolks obtained from these immunized hens, was purified using water dilution, two-step salt precipitation, and ultrafiltration. The purity of the IgY obtained was approximately 87.3 %. The antibody titer of the IgY was determined by enzyme-linked immunosorbent assay. The antibody titer peaked at 10,000 following the third immunization. In order to evaluate the inhibitory effects of the specific IgY, the growth of S. moorei in liquid media was measured every 12 h using a microplate reader at 600 nm. Biofilm formation of S. moorei was quantified by staining with crystal violet. The specific binding ability of IgY was further confirmed by the use of immunofluorescence and immunoelectron microscopy. Growth and biofilm formation of S. moorei were significantly (P<0.05) inhibited by 20 and 40 mg/ml specific IgY compared with the control. The specific IgY also decreased the bacterial level in the oral cavity of mice after infection with S. moorei. This study demonstrates that the growth and biofilm formation of S. moorei can be effectively inhibited by specific IgY. As a result, IgY technology may have application in the control of diseases caused by S. moorei.
巻・号 168(6)
ページ 1448-58
公開日 2012-11-1
DOI 10.1007/s12010-012-9869-3
PMID 22968588
MeSH Animals Antibodies, Bacterial / immunology Antibodies, Bacterial / pharmacology* Antibody Specificity Chickens Egg Yolk / immunology* Female Gram-Negative Anaerobic Bacteria / drug effects* Gram-Negative Anaerobic Bacteria / growth & development* Gram-Negative Anaerobic Bacteria / immunology Gram-Positive Bacterial Infections / immunology Gram-Positive Bacterial Infections / microbiology* Immunization Immunoglobulins / immunology Immunoglobulins / pharmacology* Mice Microbial Viability / drug effects
IF 2.277
引用数 6
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 10645 JCM 11024 JCM 12248 JCM 12257