論文 - 詳細
| RRC ID | 21472 |
|---|---|
| 著者 | Nomura H, Isshiki Y, Sakuda K, Sakuma K, Kondo S. |
| タイトル | The antibacterial activity of compounds isolated from oakmoss against Legionella pneumophila and other Legionella spp. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Oakmoss is a natural fragrance ingredient exhibiting highly specific, potent antibacterial activity against Legionella pneumophila, a causative agent of severe water-bone pneumonia. In the present study, the antibacterial activity of individual compounds isolated from oakmoss was investigated against L. pneumophila and other Legionella spp. A total of 18 known compounds and two minor novel compounds (i.e., 3-methoxy-5-methylphenyl-2,4-dihydroxy-6-methylbenzoate (compound 9) and 8-(2,4-dihydroxy-6-(2-oxoheptyl)-phenoxy)-6-hydroxy-3-pentyl-1H-isochromen-1-one (compound 20)) were purified from oakmoss. The minimum inhibitory concentrations (MICs) against clinical and environmental isolates of L. pneumophila, L. bozemanii, L. micdadei, L. longbeachae, and L. dumoffii for 11 of the 20 compounds were less than 100 µg/mL (range 0.8-64.0 µg/mL). Novel compounds 9 and 20 exhibited potent antibacterial activity against L. pneumophila strains (MIC ranges of 1.3-8.0 µg/mL and 3.3-13.3 µg/mL, respectively) and also against four other Legionella species (MIC ranges of 0.8-8.0 µg/mL and 3.3-21.3 µg/mL, respectively). Time-kill assays indicated that compounds 9 and 20 kill bacteria at a concentration equivalent to 2×MIC after 1 h and 6 h co-incubations, respectively. While oakmoss and the purified components exhibited antibacterial activity against Legionella spp., they were not active against other Gram-negative and -positive bacteria such as Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. |
| 巻・号 | 35(9) |
| ページ | 1560-7 |
| 公開日 | 2012-1-1 |
| DOI | 10.1248/bpb.b12-00452 |
| PII | DN/JST.JSTAGE/bpb/b12-00452 |
| PMID | 22975509 |
| MeSH | Anti-Bacterial Agents / pharmacology* Biological Products / isolation & purification Biological Products / pharmacology* Hydroxybenzoates / isolation & purification Hydroxybenzoates / pharmacology* Isocoumarins / isolation & purification Isocoumarins / pharmacology* Legionella / drug effects* Legionella pneumophila / drug effects* Lichens / chemistry* Microbial Sensitivity Tests |
| IF | 1.863 |
| 引用数 | 15 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 2413 JCM 5491 JCM 6119 JCM 7571 |