RRC ID 21479
Author Kim Y, Lee JW, Kang SG, Oh S, Griffiths MW.
Title Bifidobacterium spp. influences the production of autoinducer-2 and biofilm formation by Escherichia coli O157:H7.
Journal Anaerobe
Abstract The effect of Bifidobacterium spp. on the production of quorum-sensing (QS) signals and biofilm formation by enterohemorrhagic Escherichia coli (EHEC) O157:H7 was investigated. In an AI-2 bioassay, cell extracts of Bifidobacterium longum ATCC 15707 resulted in a 98-fold reduction in AI-2 activity in EHEC O157:H7 as well as in the Vibrio harveyi reporter strain, even though they did not inhibit the growth of EHEC O157:H7. In addition, they resulted in a 36% reduction in biofilm formation by the organism. Consistently, the virulence of EHEC O157:H7 was significantly attenuated by the presence of cell extracts of B. longum ATCC 15707 in the Caenorhabditis elegans nematode in vivo model. By a proteome analysis using two dimensional electrophoresis (2-DE), we determined that seven proteins including formation of iron-sulfur protein (NifU), thiol:disulfide interchange protein (DsbA), and flagellar P-ring protein (FlgI) were differentially regulated in the EHEC O157:H7 when supplemented with cell extracts of B. longum ATCC 15707. Taken together, these findings propose a novel function of a dairy adjunct in repressing the virulence of EHEC O157:H7.
Volume 18(5)
Pages 539-45
Published 2012-10
DOI 10.1016/j.anaerobe.2012.08.006
PII S1075-9964(12)00114-X
PMID 23010308
MeSH Adult Animals Anti-Bacterial Agents / isolation & purification Anti-Bacterial Agents / pharmacology* Bifidobacterium / chemistry* Biofilms / growth & development* Caenorhabditis elegans / microbiology Disease Models, Animal Escherichia coli Infections / microbiology Escherichia coli O157 / chemistry Escherichia coli O157 / drug effects* Escherichia coli O157 / pathogenicity Escherichia coli O157 / physiology* Escherichia coli Proteins / analysis Homoserine / analogs & derivatives* Homoserine / metabolism Humans Infant Lactones / metabolism* Microbial Interactions* Proteome / analysis Survival Analysis Vibrio / growth & development Vibrio / metabolism Vibrio / physiology
IF 2.704
Times Cited 16
General Microbes JCM 7052