RRC ID 21481
著者 Mokudai T, Nakamura K, Kanno T, Niwano Y.
タイトル Presence of hydrogen peroxide, a source of hydroxyl radicals, in acid electrolyzed water.
ジャーナル PLoS One
Abstract BACKGROUND:Acid electrolyzed water (AEW), which is produced through the electrolysis of dilute sodium chloride (NaCl) or potassium chloride solution, is used as a disinfectant in various fields because of its potent antimicrobial activity. The hydroxyl radical, an oxygen radical species, is often suggested as a putative active ingredient for AEW antimicrobial activity.
METHODOLOGY/PRINCIPAL FINDINGS:The aim of the present study is to detect hydroxyl radicals in AEW. The hydroxyl radicals in AEW prepared under different conditions were determined using an electron spin resonance (ESR) technique. A signal from 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH, an adduct of DMPO and the hydroxyl radical, was detected in AEW prepared by double or triple electrolyses of 1% NaCl but not of 0.1% NaCl solution. Then the presence of hydrogen peroxide as a proposed source of hydroxyl radicals was examined using a combination of ESR and a Fenton reaction. The DMPO-OH signal was clearly detected, even in AEW prepared by single electrolysis of 0.1% NaCl solution, when ferrous sulfate was added to induce a Fenton reaction, indicating the presence of hydrogen peroxide in the AEW. Since sodium formate, a hydroxyl radical scavenger, did not affect the bactericidal activity of AEW, it is concluded that the radical is unlikely to contribute to the antimicrobial activity of AEW, although a small amount of the radical is produced from hydrogen peroxide. Dimethyl sulfoxide, the other hydroxyl radical scavenger used in the present study, canceled the bactericidal activity of AEW, accompanied by complete depletion of free available chlorine, suggesting that hypochlorous acid is probably a major contributor to the antimicrobial activity.
CONCLUSIONS:It is strongly suggested that although hydrogen peroxide is present in AEW as a source of hydroxyl radicals, the antimicrobial activity of AEW does not depend on these radicals.
巻・号 7(9)
ページ e46392
公開日 2012-1-1
DOI 10.1371/journal.pone.0046392
PII PONE-D-12-20525
PMID 23029505
PMC PMC3460889
MeSH Bacillus subtilis / drug effects Bacillus subtilis / growth & development Chlorine / chemistry* Chlorine / pharmacology Cyclic N-Oxides / chemistry Dimethyl Sulfoxide / chemistry Disinfectants / chemistry* Disinfectants / pharmacology Electrolysis Electron Spin Resonance Spectroscopy Escherichia coli / drug effects Escherichia coli / growth & development Ferrous Compounds / chemistry Hydrogen Peroxide / chemistry* Hydrogen Peroxide / pharmacology Hydroxyl Radical / chemistry* Hypochlorous Acid / chemistry* Hypochlorous Acid / pharmacology Microbial Viability / drug effects Sodium Chloride / chemistry Staphylococcus aureus / drug effects Staphylococcus aureus / growth & development
IF 2.74
引用数 21
WOS 分野 FOOD SCIENCE & TECHNOLOGY
リソース情報
一般微生物 JCM 1465 JCM 2413 JCCM 5491