RRC ID 21482
Author Dicksved J, Schreiber O, Willing B, Petersson J, Rang S, Phillipson M, Holm L, Roos S.
Title Lactobacillus reuteri maintains a functional mucosal barrier during DSS treatment despite mucus layer dysfunction.
Journal PLoS ONE
Abstract Treatment with the probiotic bacterium Lactobacillus reuteri has been shown to prevent dextran sodium sulfate (DSS)-induced colitis in rats. This is partly due to reduced P-selectin-dependent leukocyte- and platelet-endothelial cell interactions, however, the mechanism behind this protective effect is still unknown. In the present study a combination of culture dependent and molecular based T-RFLP profiling was used to investigate the influence of L. reuteri on the colonic mucosal barrier of DSS treated rats. It was first demonstrated that the two colonic mucus layers of control animals had different bacterial community composition and that fewer bacteria resided in the firmly adherent layer. During DSS induced colitis, the number of bacteria in the inner firmly adherent mucus layer increased and bacterial composition of the two layers no longer differed. In addition, induction of colitis dramatically altered the microbial composition in both firmly and loosely adherent mucus layers. Despite protecting against colitis, treatment with L. reuteri did not improve the integrity of the mucus layer or prevent distortion of the mucus microbiota caused by DSS. However, L. reuteri decreased the bacterial translocation from the intestine to mesenteric lymph nodes during DSS treatment, which might be an important part of the mechanisms by which L. reuteri ameliorates DSS induced colitis.
Volume 7(9)
Pages e46399
Published 2012
DOI 10.1371/journal.pone.0046399
PII PONE-D-12-18291
PMID 23029509
PMC PMC3459901
MeSH Animals Bacterial Load Bacterial Translocation Colitis / chemically induced Colitis / microbiology Colitis / pathology Colitis / prevention & control* Colon / drug effects Colon / microbiology* Colon / pathology Dextran Sulfate Intestinal Mucosa / microbiology Intestinal Mucosa / pathology Lactobacillus reuteri / physiology* Male Metagenome / genetics* Mucus / drug effects Mucus / metabolism Mucus / microbiology* Phylogeny Polymorphism, Restriction Fragment Length Probiotics / administration & dosage Probiotics / therapeutic use* RNA, Ribosomal, 16S / genetics Rats Rats, Sprague-Dawley
IF 2.766
Times Cited 24
General Microbes JCM 5869