論文 - 詳細
| RRC ID | 21483 |
|---|---|
| 著者 | Jankowitsch F, Schwarz J, Rückert C, Gust B, Szczepanowski R, Blom J, Pelzer S, Kalinowski J, Mack M. |
| タイトル | Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. |
| ジャーナル | J Bacteriol |
| Abstract |
Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5S) and 69 tRNA genes. The 8,616 predicted protein-coding sequences include 32 clusters coding for secondary metabolites, several of which are unique to S. davawensis. The chromosome contains long terminal inverted repeats of 33,255 bp each and atypical telomeres. Sequence analysis with regard to riboflavin biosynthesis revealed three different patterns of gene organization in Streptomyces species. Heterologous expression of a set of genes present on a subgenomic fragment of S. davawensis resulted in the production of roseoflavin by the host Streptomyces coelicolor M1152. Phylogenetic analysis revealed that S. davawensis is a close relative of Streptomyces cinnabarinus, and much to our surprise, we found that the latter bacterium is a roseoflavin producer as well. |
| 巻・号 | 194(24) |
| ページ | 6818-27 |
| 公開日 | 2012-12-1 |
| DOI | 10.1128/JB.01592-12 |
| PII | JB.01592-12 |
| PMID | 23043000 |
| PMC | PMC3510588 |
| MeSH | Anti-Bacterial Agents / biosynthesis* Base Sequence Genome, Bacterial* Multigene Family Phylogeny Plasmids / genetics Riboflavin / analogs & derivatives Riboflavin / biosynthesis Sequence Analysis, DNA Streptomyces / classification Streptomyces / genetics* Streptomyces / metabolism |
| IF | 3.006 |
| 引用数 | 31 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 4913 |