Reference - Detail
| RRC ID | 21484 |
|---|---|
| Author | Mou YZ, Qiu XX, Zhao ML, Cui HL, Oh D, Dyall-Smith ML. |
| Title | Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively. |
| Journal | Extremophiles |
| Abstract |
Two halophilic archaeal strains, R30(T) and tADL(T), were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48-67 g/l MgCl(2) for growth but differ in their optimum growth temperatures (30 °C, tADL(T) vs. 40 °C, R30(T)). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB' gene similarity between strains R30(T) and tADL(T) is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4-62.9 mol % but DNA-DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30(T) (= CGMCC 1.10593(T) = JCM 17270(T)) and strain tADL(T) (= JCM 15066(T) = DSMZ 22187(T)) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively. |
| Volume | 16(6) |
| Pages | 895-901 |
| Published | 2012-11-1 |
| DOI | 10.1007/s00792-012-0485-5 |
| PMID | 23052830 |
| MeSH | Antarctic Regions Archaeal Proteins / genetics China DNA, Archaeal / chemistry Halobacteriaceae / classification* Halobacteriaceae / cytology Halobacteriaceae / isolation & purification* Phylogeny RNA, Ribosomal, 16S / genetics |
| IF | 2.462 |
| Times Cited | 26 |
| WOS Category | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 9738 JCM 15066 JCM 17270 |