| RRC ID |
21488
|
| Author |
Jojima T, Igari T, Gunji W, Suda M, Inui M, Yukawa H.
|
| Title |
Identification of a HAD superfamily phosphatase, HdpA, involved in 1,3-dihydroxyacetone production during sugar catabolism in Corynebacterium glutamicum.
|
| Journal |
FEBS Lett
|
| Abstract |
Corynebacterium glutamicum produces 1,3-dihydroxyacetone (DHA) as metabolite of sugar catabolism but the responsible enzyme is yet to be identified. Using a transposon mutant library, the gene hdpA (cgR_2128) was shown to encode a haloacid dehalogenase superfamily member that catalyzes dephosphorylation of dihydroxyacetone phosphate to produce DHA. Inactivation of hdpA led to a drastic decrease in DHA production from each of glucose, fructose, and sucrose, indicating that HdpA is the main enzyme responsible for DHA production from sugars in C. glutamicum. Confirmation of DHA production via dihydroxyacetone phosphatase finally confirms a long-speculated route through which bacteria produce DHA.
|
| Volume |
586(23)
|
| Pages |
4228-32
|
| Published |
2012-11-30
|
| DOI |
10.1016/j.febslet.2012.10.028
|
| PII |
S0014-5793(12)00811-3
|
| PMID |
23108048
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Corynebacterium glutamicum / enzymology*
Dihydroxyacetone / biosynthesis*
Dihydroxyacetone / metabolism
Phosphoric Monoester Hydrolases / genetics
Phosphoric Monoester Hydrolases / metabolism*
|
| IF |
3.057
|
| Times Cited |
16
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| General Microbes |
JCM 18229 |
