| RRC ID |
21514
|
| Author |
Yamaguchi K, Feril LB Jr, Tachibana K, Takahashi A, Matsuo M, Endo H, Harada Y, Nakayama J.
|
| Title |
Ultrasound-mediated interferon β gene transfection inhibits growth of malignant melanoma.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We investigated the effects of ultrasound-mediated transfection (sonotransfection) of interferon β (IFN-β) gene on melanoma (C32) both in vitro and in vivo. C32 cells were sonotransfected with IFN-β in vitro. Subcutaneous C32 tumors in mice were sonicated weekly immediately after intra-tumor injection with IFN-β genes mixed with microbubbles. Successful sonotransfection with IFN-β gene in vitro was confirmed by ELISA, which resulted in C32 growth inhibition. In vivo, the growth ratio of tumors transfected with IFN-β gene was significantly lower than the other experimental groups. These results may lead to a new method of treatment against melanoma and other hard-to-treat cancers.
|
| Volume |
411(1)
|
| Pages |
137-42
|
| Published |
2011-7-22
|
| DOI |
10.1016/j.bbrc.2011.06.115
|
| PII |
S0006-291X(11)01095-3
|
| PMID |
21723255
|
| MeSH |
Animals
Cell Line, Tumor
Female
Genetic Therapy / methods*
Humans
Interferon-beta / genetics*
Interferon-beta / metabolism
Melanoma / therapy*
Mice
Mice, Inbred BALB C
Mice, Nude
Skin Neoplasms / genetics*
Transfection / methods*
Ultrasonics
|
| IF |
2.985
|
| Times Cited |
12
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pCMV (Hu beta) (RDB01681) |
