| RRC ID |
21520
|
| Author |
Chen Q, Osada K, Ishii T, Oba M, Uchida S, Tockary TA, Endo T, Ge Z, Kinoh H, Kano MR, Itaka K, Kataoka K.
|
| Title |
Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors.
|
| Journal |
Biomaterials
|
| Abstract |
Homo-poly{N'-[N-(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H] was attempted to integrate into poly (ethylene glycol) (PEG)-b-PAsp(DET)] (B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration. In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape. In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression. These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
|
| Volume |
33(18)
|
| Pages |
4722-30
|
| Published |
2012-6-1
|
| DOI |
10.1016/j.biomaterials.2012.03.017
|
| PII |
S0142-9612(12)00289-X
|
| PMID |
22444644
|
| MeSH |
Animals
Cell Line
Cell Survival / drug effects
Flow Cytometry
Genetic Therapy / methods*
Humans
Mice
Mice, Nude
Micelles*
Microscopy, Electron, Transmission
Nanoparticles / adverse effects
Nanoparticles / chemistry*
Pancreatic Neoplasms / therapy*
Polyethylene Glycols / chemistry*
|
| IF |
10.317
|
| Times Cited |
40
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| DNA material |
pCAcc-Luc+ (RDB02443) |
