Reference - Detail
| RRC ID | 21524 |
|---|---|
| Author | Girdwood D, Robertson M, Gordon C. |
| Title | Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. |
| Journal | FEBS Lett |
| Abstract |
In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability. |
| Volume | 586(10) |
| Pages | 1522-8 |
| Published | 2012-5-21 |
| DOI | 10.1016/j.febslet.2012.04.011 |
| PII | S0014-5793(12)00289-X |
| PMID | 22673520 |
| MeSH | Base Sequence Cullin Proteins / physiology* DNA Primers Ligases / metabolism* Molecular Sequence Data Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 3.057 |
| Times Cited | 2 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| DNA material | pDUAL-FFH1c (RDB06142) S. pombe FLAG2-His SpFFH20F06 (SPW048126) SpFFH47B10 (SPW058834) |