RRC ID 21525
著者 Tanaka Y, Sato Y, Osawa S, Inoue M, Tanaka S, Sasaki T.
タイトル Suppression of feline coronavirus replication in vitro by cyclosporin A.
ジャーナル Vet Res
Abstract The feline infectious peritonitis virus (FIPV) is a member of the feline coronavirus family that causes FIP, which is incurable and fatal in cats. Cyclosporin A (CsA), an immunosuppressive agent that targets the nuclear factor pathway of activated T-cells (NF-AT) to bind cellular cyclophilins (CyP), dose-dependently inhibited FIPV replication in vitro. FK506 (an immunosuppressor of the pathway that binds cellular FK506-binding protein (FKBP) but not CyP) did not affect FIPV replication. Neither cell growth nor viability changed in the presence of either CsA or FK506, and these factors did not affect the NF-AT pathway in fcwf-4 cells. Therefore, CsA does not seem to exert inhibitory effects via the NF-AT pathway. In conclusion, CsA inhibited FIPV replication in vitro and further studies are needed to verify the practical value of CsA as an anti-FIPV treatment in vivo.
巻・号 43(1)
ページ 41
公開日 2012-4-30
DOI 10.1186/1297-9716-43-41
PII 1297-9716-43-41
PMID 22546085
PMC PMC3403912
MeSH Animals Blotting, Western / veterinary Cats Cell Line Coronavirus, Feline / physiology* Cyclosporine / pharmacology* Dose-Response Relationship, Drug Feline Infectious Peritonitis / virology* Immunosuppressive Agents / pharmacology* NFATC Transcription Factors / genetics* NFATC Transcription Factors / metabolism Nucleocapsid Proteins / genetics Nucleocapsid Proteins / metabolism Polymerase Chain Reaction / veterinary Tacrolimus / pharmacology* Virus Replication / drug effects*
IF 3.357
引用数 21
WOS 分野 VETERINARY SCIENCES
リソース情報
遺伝子材料 pISRE-TK hRluc(F) (RDB05610)