RRC ID 21539
Author Egawa N, Nakahara T, Ohno S, Narisawa-Saito M, Yugawa T, Fujita M, Yamato K, Natori Y, Kiyono T.
Title The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome.
Journal J Virol
Abstract Papillomavirus genomes are thought to be amplified to about 100 copies per cell soon after infection, maintained constant at this level in basal cells, and amplified for viral production upon keratinocyte differentiation. To determine the requirement for E1 in viral DNA replication at different stages, an E1-defective mutant of the human papillomavirus 16 (HPV16) genome featuring a translation termination mutation in the E1 gene was used. The ability of the mutant HPV16 genome to replicate as nuclear episomes was monitored with or without exogenous expression of E1. Unlike the wild-type genome, the E1-defective HPV16 genome became established in human keratinocytes only as episomes in the presence of exogenous E1 expression. Once established, it could replicate with the same efficiency as the wild-type genome, even after the exogenous E1 was removed. However, upon calcium-induced keratinocyte differentiation, once again amplification was dependent on exogenous E1. These results demonstrate that the E1 protein is dispensable for maintenance replication but not for initial and productive replication of HPV16.
Volume 86(6)
Pages 3276-83
Published 2012-3-1
DOI 10.1128/JVI.06450-11
PII JVI.06450-11
PMID 22238312
PMC PMC3302310
MeSH Cell Line DNA Replication Genome, Viral* Human papillomavirus 16 / genetics Human papillomavirus 16 / physiology* Humans Oncogene Proteins, Viral / genetics Oncogene Proteins, Viral / metabolism* Papillomavirus Infections / virology* Virus Replication*
IF 4.501
Times Cited 43
DNA material AxCANCre (RDB01748)