RRC ID 21541
Author Yamagishi M, Matsubara K, Sakaizumi M.
Title Molecular cytogenetic identification and characterization of Robertsonian chromosomes in the large Japanese field mouse (Apodemus speciosus) using FISH.
Journal Zoolog Sci
Abstract Robertsonian (Rb) karyotypic polymorphism in Apodemus speciosus has interested many researchers with particular referece to the genetic divergence between Rb and non-Rb populations. Failure to find morphologic, biochemical, or genetic differences in previous studies reveals the necessity of focusing on loci on Rb chromosomes, which can be characterized by FISH mapping with DNA probes. In an Rb heterozygote, DNA probes from laboratory mouse chromosomes (MMUs) 1 and 10 were simultaneously hybridized to the long arm of a metacentric and a medium-sized acrocentric chromosome and to the short arm of the metacentric and a small acrocentric chromosome, respectively. Four additional probes derived from each of MMUs 1 and 10 were mapped to the long and short arms, respectively, of the Rb chromosome identified by the above markers. Homologies between the long arm of the Rb chromosome and MMU 1 and between the short arm and MMU 10 were supported by all ten markers, which were dispersed along nearly the entire lengths of the Rb chromosomes. These results indicate that the long and short arms of the Rb chromosomes are homologous to Apodemus speciosus chromosomes 12 and 19 (defined in a previous study), respectively. This ten-marker series can be useful for detecting chromosome-specific divergence between the two karyotypic populations at the gene level.
Volume 29(10)
Pages 709-13
Published 2012-10-1
DOI 10.2108/zsj.29.709
PMID 23030344
MeSH Animals Chromosomes / classification* Cytogenetics Genetic Markers Genetic Variation In Situ Hybridization, Fluorescence / veterinary* Karyotype Murinae / genetics* Translocation, Genetic*
IF 0.843
Times Cited 1
DNA material MSM Mouse BAC (RDB04214) MSMg01-471G06 MSMg01-400O15 MSMg01-134B19 MSMg01-333O13 MSMg01-500L10 MSMg01-528O02 B6N BAC Mouse (RDB07573) B6Ng01-004K19 B6Ng01-267N11 B6Ng01-280P05 B6Ng01-306H11.