RRC ID 21550
Author Fujii M, Yokosho K, Yamaji N, Saisho D, Yamane M, Takahashi H, Sato K, Nakazono M, Ma JF.
Title Acquisition of aluminium tolerance by modification of a single gene in barley.
Journal Nat Commun
Abstract Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.
Volume 3
Pages 713
Published 2012-3-6
DOI 10.1038/ncomms1726
PII ncomms1726
PMID 22395604
PMC PMC3316887
MeSH Adaptation, Physiological / genetics Aluminum / metabolism Aluminum / toxicity* Base Sequence Biological Transport Carrier Proteins / genetics* Citric Acid / metabolism Gene Expression Regulation, Plant Genes, Plant Hordeum / enzymology Hordeum / genetics* Hordeum / metabolism Hydrogen-Ion Concentration Iron / metabolism Molecular Sequence Data Mutagenesis, Insertional Plant Roots / metabolism Plants, Genetically Modified Rhizosphere Sequence Analysis, DNA Soil / chemistry Xylem / metabolism
IF 12.121
Times Cited 95
WOS Category PLANT SCIENCES
Resource
Barley