RRC ID 21595
著者 Neilan BA, Jacobs D, Goodman AE.
タイトル Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus.
ジャーナル Appl Environ Microbiol
Abstract Cyanobacteria are a highly diverse group in relation to form, function, and habitat. Current cyanobacterial systematics relies on the observation of minor and plastic morphological characters. Accurate and reliable delineation of toxic and bloom-forming strains of cyanobacteria has not been possible by traditional methods. We have designed general primers to the phycocyanin operon (cpc gene) and developed a PCR which allows the amplification of a region of this gene, including a variable intergenic spacer sequence. Because of the specificity of this PCR for cyanobacterial isolates, the assay is appropriate for the rapid and reliable identification of strains in freshwater samples. Successive restriction endonuclease digestion of this amplification product, with a total of nine enzymes, yielded many identifying DNA profiles specific to the various taxonomic levels of cyanobacteria. The restriction enzyme profiles for MspI, RsaI, and TaqI were conserved for strains within each of the eight genera (40 strains) studied and clearly discriminated among these genera. Intrageneric delineation of strains was revealed by the enzymes AluI, CfoI, and HaeIII for members of the genus Microcystis, while strains of genus Anabaena were differentiated by the digestion patterns provided by AluI, CfoI, and ScrFI. Phenetic and cladistic analyses of the data were used to infer the genetic relatedness and evolution of toxic and bloom-forming cyanobacteria.
巻・号 61(11)
ページ 3875-83
公開日 1995-11-1
DOI 10.1128/aem.61.11.3875-3883.1995
PMID 8526499
PMC PMC167692
MeSH Base Sequence Cyanobacteria / classification Cyanobacteria / genetics* Cyanobacteria / pathogenicity DNA Primers / genetics DNA, Bacterial / genetics* Genetic Variation Molecular Sequence Data Phycocyanin / genetics* Phylogeny Polymerase Chain Reaction Polymorphism, Genetic* Polymorphism, Restriction Fragment Length Species Specificity
IF 4.016
引用数 228
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
藻類 NIES-24 NIES-40 NIES-41 NIES-44 NIES-73 NIES-76 NIES-78 NIES-80 NIES-81 NIES-87 NIES-89 NIES-98 NIES-102 NIES-107 NIES-111 NIES-112 NIES-204 NIES-207 NIES-298