| RRC ID |
21619
|
| Author |
Yamaguchi M, Ogawa T, Muramoto K, Kamio Y, Jimbo M, Kamiya H.
|
| Title |
Isolation and characterization of a mannan-binding lectin from the freshwater cyanobacterium (blue-green algae) Microcystis viridis.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Microcystis viridis NIES-102 strain, a unicellular freshwater bloom-forming cyanobacterium, showed transient hemagglutinating activity in laboratory culture during stationary phase under nonaeration conditions. However, the hemagglutinating activity which was inhibited with yeast mannan could not be observed during culture with aeration. A mannan-binding lectin named MVL was isolated with the assay of the hemagglutinating activity against rabbit erythrocytes from the cyanobacterium by successive hydrophobic and gel filtration chromatography. MVL was composed of a single polypeptide of 13 kDa. The gene (mvl) for MVL was cloned from a genomic DNA of NIES-102 strain as a template, and its sequence was determined. The deduced amino acid sequence showed that MVL consisted of 113 amino acid residues and was composed of two tandemly repeated homologous domains of 54 amino acid residues. MVL showed no sequence homology to any other lectins or proteins.
|
| Volume |
265(3)
|
| Pages |
703-8
|
| Published |
1999-11-30
|
| DOI |
10.1006/bbrc.1999.1749
|
| PII |
S0006-291X(99)91749-7
|
| PMID |
10600484
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins / genetics
Carrier Proteins / isolation & purification*
Carrier Proteins / metabolism
Collectins
DNA Primers / genetics
DNA, Bacterial / genetics
Genes, Bacterial
Hemagglutination Tests
In Vitro Techniques
Lectins / genetics
Lectins / isolation & purification*
Lectins / metabolism
Mannans / metabolism*
Microcystis / genetics
Microcystis / metabolism*
Molecular Sequence Data
Molecular Weight
Rabbits
Repetitive Sequences, Amino Acid
Sequence Homology, Amino Acid
|
| IF |
2.985
|
| Times Cited |
39
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Algae |
NIES-102 |
