RRC ID 21732
Author Mizuno Y, Sato A, Watanabe K, Hirata A, Takeshita T, Ota S, Sato N, Zachleder V, Tsuzuki M, Kawano S.
Title Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species.
Journal Bioresour Technol
Abstract The influence of sulfur deficiency on biomass production was analyzed in the four Chlorellaceae species, Chlorella vulgaris, Chlorella sorokiniana, Chlorella lobophora, and Parachlorella kessleri. Culturing under sulfur-deficient conditions promoted transient accumulation of starch followed by a steady increase in lipid storage. Transmission electron microscopy indicated an increase and decrease in starch granules and subsequent enlargement of lipid droplets under sulfur-deficient conditions. Chlorellaceae spp. accumulated 1.5-2.7-fold higher amounts of starch and 1.5-2.4-fold higher amounts of lipid under sulfur-deficient conditions than under sulfur-sufficient conditions. More than 75% of the fatty acids that accumulated in Chlorellaceae spp. under the sulfur-sufficient condition were unsaturated and culturing under sulfur-deficient conditions increased the saturated fatty acid content from 24.3% to 59.7% only in P. kessleri. These results indicate that the sequential accumulation of starch and lipid is a response to the sulfur depletion that commonly occurs in Chlorellaceae spp.
Volume 129
Pages 150-5
Published 2013-2-1
DOI 10.1016/j.biortech.2012.11.030
PII S0960-8524(12)01704-X
PMID 23238344
MeSH Bioreactors / microbiology* Chlorella / classification* Chlorella / metabolism* Lipid Metabolism / physiology* Species Specificity Starch / isolation & purification Starch / metabolism* Sulfur / metabolism*
IF 7.539
Times Cited 53
Algae NIES-2169 NIES-2173