論文 - 詳細
| RRC ID | 21761 |
|---|---|
| 著者 | Fujise D, Tsuji K, Fukushima N, Kawai K, Harada K. |
| タイトル | Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior. |
| ジャーナル | J Chromatogr A |
| Abstract |
In order to fully understand the role of volatile organic compounds (VOCs) under natural conditions, an adaptable analytical method was developed as the first step. beta-Ionone, beta-cyclocitral, 2-methyl-1-butanol and 3-methyl-1-butanol were simultaneously analyzed in addition to geosmin and 2-MIB using GC/MS with SPME. The slight modification of a known method allowed the simultaneous detection and quantification of these VOCs. The SIM of the 3-methyl-1-butanol was always accompanied by a shoulder peak, suggesting the presence of two compounds. In order to separate both compounds, the GC/MS conditions were optimized, and the additional peak was identified as 2-methyl-1-butanol by direct comparison of the authentic compound, indicating that the Microcystis strain always produces a mixture of 2-methyl-1-butanol and 3-methyl-1-butanol. Furthermore, it was found that 2-methyl-1-butanol and 3-methyl-1-butanol were predominant in the dissolved fractions. beta-Cyclocitral was easily oxidized to provide the oxidation product, 2,6,6-trimethylcyclohexene-1-carboxylic acid, which causes the blue color formation of cyanobacteria as a consequence of acid stress. The intact acid could be satisfactorily analyzed using the usual GC/MS without derivatization. |
| 巻・号 | 1217(39) |
| ページ | 6122-5 |
| 公開日 | 2010-9-24 |
| DOI | 10.1016/j.chroma.2010.07.008 |
| PII | S0021-9673(10)00893-9 |
| PMID | 20797719 |
| MeSH | Butanols / chemistry Cyanobacteria / chemistry* Fresh Water / chemistry Gas Chromatography-Mass Spectrometry / methods* Solid Phase Microextraction / methods* Volatile Organic Compounds / analysis* Volatile Organic Compounds / chemistry Volatile Organic Compounds / isolation & purification |
| IF | 4.049 |
| 引用数 | 24 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 藻類 | NIES-87 NIES-298 |