RRC ID 21783
著者 Sano D, Ishifuji S, Sato Y, Imae Y, Takaara T, Masago Y, Omura T.
タイトル Identification and characterization of coagulation inhibitor proteins derived from cyanobacterium Microcystis aeruginosa.
ジャーナル Chemosphere
Abstract The excess growth of cyanobacteria in semi enclosed water areas caused by eutrophication brings about coagulation inhibition in drinking water treatment processes. In this study, coagulation inhibitor proteins produced by Microcystis aeruginosa, a major cyanobacterium in algal bloom, were acquired by a phage display technique, an aluminum-immobilized affinity chromatography and a protein expression technique using Escherichia coli cells. Two cyanobacterial peptides with a high ratio of metallophilic amino acids were recovered, which were a part of homologues of a thiol oxidase enzyme Ero1p and a trans-acting repressor ArsR. It was also shown that the homologue of ArsR exhibited a stronger inhibitory effect on the coagulation of kaolin suspension with polyaluminum chloride than the control proteins. This is the first report to identify a cyanobacterial cell component to inhibit coagulation. The compositions of polar amino acids were critical to explain the strength of coagulation inhibition potential. Polar proteins from cyanobacteria could collectively consume coagulants or stabilize clay particles, which would be plausible explanations for causing coagulation inhibition. Meanwhile, results from the kaolin coagulation tests using the control proteins implied that the neutralization of positive charges of coagulant constituents by simple electrostatic interactions might not be the key mechanism on the protein-induced coagulation inhibition.
巻・号 82(8)
ページ 1096-102
公開日 2011-2-1
DOI 10.1016/j.chemosphere.2010.12.005
PII S0045-6535(10)01395-0
PMID 21211815
MeSH Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Eutrophication Kaolin / metabolism Microcystis / growth & development Microcystis / metabolism* Water Purification Water Supply / analysis*
IF 5.778
引用数 19
WOS 分野 ENVIRONMENTAL SCIENCES
リソース情報
藻類 NIES-91