RRC ID |
2180
|
著者 |
Sato M, Kong CJ, Yoshida H, Nakamura T, Wada A, Shimoda C, Kaneda Y.
|
タイトル |
Ribosomal proteins S0 and S21 are involved in the stability of 18S rRNA in fission yeast, Schizosaccharomyces pombe.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Stability of ribosomal RNA (rRNA) is not only essential for ribosome biogenesis but also crucial to the maintenance of proper translational level for cell viability. rRNA processing (maturation) is one of the key steps to derive functional rRNA, and to date, a large number of factors involved in this process have been identified. We investigated Rps0 binding proteins in fission yeast, Schizosaccharomyces pombe, and revealed that Rps0p is associated with Rps21 protein, similar to that of our previous observation in human cells. We demonstrated that both rps0(+)s and rps21(+) are essential genes for S. pombe analyzed by tetrad dissection assay. To study the functions of both genes, we established disruption strains transformed with inducible rescue plasmids. Using the strains our studies revealed that the loss of rps0(+)s or rps21(+) led to a deficiency of 40S ribosomal subunit formation. Additional functional studies indicate that this phenomenon is likely to be caused by insufficient 18S rRNA stability. The possible role of Rps0p and Rps21 that contribute to 18S rRNA maturation is further discussed.
|
巻・号 |
311(4)
|
ページ |
942-7
|
公開日 |
2003-11-28
|
DOI |
10.1016/j.bbrc.2003.10.086
|
PII |
S0006291X03021661
|
PMID |
14623272
|
MeSH |
Protein Precursors / genetics
Protein Precursors / metabolism*
RNA Stability / genetics
RNA Stability / physiology*
RNA, Ribosomal, 18S / genetics
RNA, Ribosomal, 18S / metabolism*
Receptors, Laminin / genetics
Receptors, Laminin / metabolism*
Ribosomal Proteins / genetics
Ribosomal Proteins / metabolism
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
|
IF |
2.985
|
引用数 |
8
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
酵母 |
L972
YH100-1A
SFY526 |