RRC ID 2188
Author Tougan T, Chiba Y, Kakihara Y, Hirata A, Nojima H.
Title Meu10 is required for spore wall maturation in Schizosaccharomyces pombe.
Journal Genes Cells
Abstract BACKGROUND:Many genes are meiosis and/or sporulation-specifically transcribed during this process. Isolation and analysis of these genes might help us to understand how meiosis and sporulation are regulated. For this purpose, we have isolated a large number of cDNA clones from Schizosaccharomyces pombe whose expression is up-regulated during meiosis.
RESULTS:We have isolated meu10+ gene, which encodes 416 amino acids and bears homology to SPS2 of Saccharomyces cerevisiae. A strain whose meu10+ gene has been deleted forms no viable spores. Thin-section electron micrographs showed that the meu10Delta strain has abnormally formed spore walls, and then they disrupt, allowing cytoplasmic material to escape. The Meu10-GFP fusion protein is localized to the spore periphery, thereafter returned to the cytoplasm after sporulation. Meu10-GFP localization to the spore wall was almost normal in the bgs2Delta or chs1Delta mutants that lack 1,3-beta-glucan or chitin, respectively. In contrast, 1,3-beta-glucan is abnormally localized in meu10Delta cells. Meu10 has an N-terminal domain with homology to the mammalian insulin receptor and a C-terminal domain with a transmembrane motif. Mutants whose N-terminal or C-terminal domain was truncated were severely defective for sporulation.
CONCLUSIONS:Meu10 is a spore wall component and plays a pivotal role in the formation of the mature spore wall structure.
Volume 7(2)
Pages 217-31
Published 2002-2-1
DOI 10.1046/j.1356-9597.2001.00511.x
PII 511
PMID 11895484
MeSH Amino Acid Sequence Fungal Proteins / genetics* Fungal Proteins / physiology Molecular Sequence Data Phylogeny Schizosaccharomyces / genetics Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / physiology Sequence Alignment Sequence Deletion / genetics Spores, Fungal / physiology* Spores, Fungal / ultrastructure
IF 1.655
Times Cited 27
Yeast FY9907(TP4-5A) FY9906(TP4-1D) CD16-1 FY7051(JZ670) FY7476(AB4) FY16902(YCM101) FY16903(YCM102) FY16905(TTM102)