Reference - Detail
RRC ID | 2188 |
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Author | Tougan T, Chiba Y, Kakihara Y, Hirata A, Nojima H. |
Title | Meu10 is required for spore wall maturation in Schizosaccharomyces pombe. |
Journal | Genes Cells |
Abstract |
BACKGROUND:Many genes are meiosis and/or sporulation-specifically transcribed during this process. Isolation and analysis of these genes might help us to understand how meiosis and sporulation are regulated. For this purpose, we have isolated a large number of cDNA clones from Schizosaccharomyces pombe whose expression is up-regulated during meiosis. RESULTS:We have isolated meu10+ gene, which encodes 416 amino acids and bears homology to SPS2 of Saccharomyces cerevisiae. A strain whose meu10+ gene has been deleted forms no viable spores. Thin-section electron micrographs showed that the meu10Delta strain has abnormally formed spore walls, and then they disrupt, allowing cytoplasmic material to escape. The Meu10-GFP fusion protein is localized to the spore periphery, thereafter returned to the cytoplasm after sporulation. Meu10-GFP localization to the spore wall was almost normal in the bgs2Delta or chs1Delta mutants that lack 1,3-beta-glucan or chitin, respectively. In contrast, 1,3-beta-glucan is abnormally localized in meu10Delta cells. Meu10 has an N-terminal domain with homology to the mammalian insulin receptor and a C-terminal domain with a transmembrane motif. Mutants whose N-terminal or C-terminal domain was truncated were severely defective for sporulation. CONCLUSIONS:Meu10 is a spore wall component and plays a pivotal role in the formation of the mature spore wall structure. |
Volume | 7(2) |
Pages | 217-31 |
Published | 2002-2-1 |
DOI | 10.1046/j.1356-9597.2001.00511.x |
PII | 511 |
PMID | 11895484 |
MeSH | Amino Acid Sequence Fungal Proteins / genetics* Fungal Proteins / physiology Molecular Sequence Data Phylogeny Schizosaccharomyces / genetics Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / physiology Sequence Alignment Sequence Deletion / genetics Spores, Fungal / physiology* Spores, Fungal / ultrastructure |
IF | 1.655 |
Times Cited | 27 |
WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
Resource | |
Yeast | FY9907(TP4-5A) FY9906(TP4-1D) CD16-1 FY7051(JZ670) FY7476(AB4) FY16902(YCM101) FY16903(YCM102) FY16905(TTM102) |