| RRC ID |
21922
|
| Author |
Tani S, Nakagawa K, Honda T, Saito H, Suzuki Y, Kawamata J, Uchida M, Sasaki A, Kinjo M.
|
| Title |
Fluorescence imaging of mitochondria in living cells using a novel fluorene derivative with a large two-photon absorption cross-section.
|
| Journal |
Curr Pharm Biotechnol
|
| Abstract |
We have examined optical properties of a fluorene derivative with two positively charged substituents, 1,1'- diethyl-4,4'-(9,9-diethyl-2,7-fluorenediyl-2,1-ethanediyl)dipyridinium perchlorate (1), in water. The photoluminescence quantum yield of 1 was relatively high (35%) for use as a fluorescent probe in water. We also examined two-photon absorption (TPA) properties of 1 in methanol. The maximum value of the TPA cross-section (730 GM at 750 nm, 1 GM = 10(-50) cm4 s photon-1 molecule-1) was larger than that for most two-photon-excited fluorescent dyes including a classical mitochondria-selective fluorescent dye rhodamine 123. Preliminary fluorescence imaging experiments of the mitochondria in living Paramecium caudatum and HeLa cells were carried out with 1. Bright green fluorescence was observed from the mitochondria in both living cells loaded 1 without toxicity effects. These our results indicate that water-soluble fluorene derivative 1 is a promising candidate as a two-photon-excited fluorescence probe for mitochondria in living cells.
|
| Volume |
13(14)
|
| Pages |
2649-54
|
| Published |
2012-12-18
|
| DOI |
10.2174/138920101314151120122918
|
| PII |
BSP/CPB/E-Pub/0000142
|
| PMID |
22039816
|
| MeSH |
Absorption
Fluorenes / chemistry
Fluorenes / pharmacology*
Fluorescent Dyes / pharmacology
HeLa Cells
Humans
Indoles / pharmacology
Microscopy, Fluorescence
Mitochondria / metabolism*
Paramecium caudatum / metabolism*
Photons
|
| IF |
2.097
|
| Times Cited |
7
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Paramecium |
KNZ1209 (PC011014A) |
